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Species: Other
Genetic Insert: INU1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125039 Copy
Species: Mus musculus
Genetic Insert: HH5-5 TCRa P2A TCRb
Vector Backbone Description: Vector Backbone:pMigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124985 Copy
Species: Synthetic
Genetic Insert: Luc2, copGFP
Vector Backbone Description: Vector Backbone:pCDH-EF1 (Addgene #72266); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124982 Copy
Species: Other
Genetic Insert: TDH1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part
Please note: Addgene NGS finds two ambiguous bases within the KmTDH1pr sequence. It is not known if these regions contain regulatory elements. Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125037 Copy
Species: Mus musculus
Genetic Insert: TCR-HH5-3
Vector Backbone Description: Vector Backbone:pMigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124983 Copy
Species: Synthetic
Genetic Insert: SO_0622(REC)-psdR(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125097 Copy
Species: Synthetic
Genetic Insert: SO_2193(REC)-psdR(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125098 Copy
Species: Homo sapiens
Genetic Insert: FOXO2
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247077
Comments: Expression vector for Akt-FoxO3a-KTR-EGFP in mammalian cells.
Proper citation: RRID:Addgene_125131 Copy
Species: Synthetic
Genetic Insert: torR(REC)-psdR(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125095 Copy
Species: Synthetic
Genetic Insert: torR(REC)-yxdJ(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125096 Copy
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125134 Copy
Species: Synthetic
Genetic Insert: SO_4388(REC)-psdR(DBD)137
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125099 Copy
Species: Other
Genetic Insert: mNeon/mScarletP2A Rac1-V12
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Rac1-V12 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged Rac1-V12.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125136 Copy
Species: Other
Genetic Insert: mNeon/mScarletP2A Rac1-N17
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Rac1-N17 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged Rac1-N17.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125137 Copy
Species: Synthetic
Genetic Insert: ompR(REC)-manR(DBD)122
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125093 Copy
Species: Synthetic
Genetic Insert: ompR(REC)-psdR(DBD)122
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125092 Copy
Species: Synthetic
Genetic Insert: ompR(REC)-ccaR(DBD)150
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125084 Copy
Species: Synthetic
Genetic Insert: ompR(REC)-ccaR(DBD)110
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125085 Copy
Species: Mammalian
Genetic Insert: iNicSnFR3b-(V7)-PM
Vector Backbone Description: Backbone Marker:Janelia; Backbone Size:5005; Vector Backbone:pMinDis.X513; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30718376
Comments: Please note that a 6bp insertion was identified in the NeoR/KanR cassette during Addgene's quality control process. We recommend testing NeoR selection before performing experiments. Vector was received from Janelia HHMI.
Proper citation: RRID:Addgene_125124 Copy
Species: Synthetic
Genetic Insert: ompR(REC)-ccaR(DBD)139
Vector Backbone Description: Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125088 Copy
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