Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Vibrio fischeri
Genetic Insert: luxR
Vector Backbone Description: Vector Backbone:pGK415; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Proper citation: RRID:Addgene_165971 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (4xTBE1 wt)
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9727977
Comments: Tcf-4 binding elements (TBEs). To construct the four tandem repeats of TBE1, we dimerized oligonucleotide cassettes containing two copies of the site and cloned the products into pBV-Luc (5'-CTAGCGCACCTTTGATTTCTGCACCTTTGATTTCTG-3' and 5'-CTAGCAGAAATCAAAGGTGCAGAAATCAAAGGTGCG-3')
Proper citation: RRID:Addgene_16597 Copy
Species: Homo sapiens
Genetic Insert: p53 binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: The following oligo pairs containing two copies of wild-type p53 binding sites were used: 5'-CTAGGCTGCA AGTCCTGACT TGTCCACACT CTGCAAGTCC TGACTTGTCC-3' and 5'-CTAGGGACAA GTCAGGACTT GCAGAGTGTG GACAAGTCAG GACTTGCAGC-3'. The annealed oligonucleotide pairs were concatamerized and cloned into the NheI site of pBVLuc.
Proper citation: RRID:Addgene_16593 Copy
Species: Homo sapiens
Genetic Insert: PUMA promotor fragment
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: Deletion of 5' terminal 300 bp from Frag1. Does not contain consensus p53 binding sites BS1 and BS2.
Proper citation: RRID:Addgene_16592 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Comments: A portion of this plasmid (the operator region of ptetO7) was derived from a plasmid (pTRE-Tight, Tet systems).
Proper citation: RRID:Addgene_165976 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Proper citation: RRID:Addgene_165977 Copy
Species: Synthetic
Genetic Insert: Basic Selection Vector
Vector Backbone Description: Backbone Size:2327; Vector Backbone:pColE1_Alpha2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha2
Proper citation: RRID:Addgene_165882 Copy
Species: Synthetic
Genetic Insert: Transgenesis Ready Selection Vector
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pColE1_AlphaOmega; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34525356
Comments: Terminal Alpha Entry
Proper citation: RRID:Addgene_165883 Copy
Species: Synthetic
Genetic Insert: All-in-one FC31 RMCE Cassette
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega1
Proper citation: RRID:Addgene_165887 Copy
Species: Synthetic
Genetic Insert: Selectable QUAS Response Vector
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pColE1_AlphaOmega; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34525356
Comments: Terminal Alpha Entry
Proper citation: RRID:Addgene_165920 Copy
Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD130.07, Ligation number L4507.
Proper citation: RRID:Addgene_1659 Copy
Species: Synthetic
Genetic Insert: Selectable QUAS Response Vector
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pColE1_AlphaOmega; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34525356
Comments: Terminal Alpha Entry
Proper citation: RRID:Addgene_165924 Copy
Species: Synthetic
Genetic Insert: All-in-one FC31 RMCE Cassette
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega1
Proper citation: RRID:Addgene_165888 Copy
Species: Synthetic
Genetic Insert: Selectable QUAS Response Vector
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pColE1_AlphaOmega; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34525356
Comments: Terminal Alpha Entry
Proper citation: RRID:Addgene_165921 Copy
Species: Synthetic
Genetic Insert: All-in-one FC31 RMCE Cassette
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: Omega1
Proper citation: RRID:Addgene_165889 Copy
Species: Synthetic
Genetic Insert: Selectable QUAS Response Vector
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pColE1_AlphaOmega; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34525356
Comments: Terminal Alpha Entry
Proper citation: RRID:Addgene_165922 Copy
Species: Synthetic
Genetic Insert: Eye Marker Cassette
Vector Backbone Description: Backbone Size:2287; Vector Backbone:pColE1_Alpha1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha1
Proper citation: RRID:Addgene_165872 Copy
Species: Synthetic
Genetic Insert: DAD2 cPGFP(G164)
Vector Backbone Description: Backbone Size:5717; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32786922
Proper citation: RRID:Addgene_165993 Copy
Species: Synthetic
Genetic Insert: SpyCatcher-M2e
Vector Backbone Description: Backbone Size:5676; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33468139
Comments: Original publications:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.
Proper citation: RRID:Addgene_165990 Copy
Species: Synthetic
Genetic Insert: Eye Marker Cassette
Vector Backbone Description: Backbone Size:2327; Vector Backbone:pColE1_Alpha2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha2
Proper citation: RRID:Addgene_165875 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.