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Species: Saguinus labiatus (red-chested mustached tamarin)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45378 Copy
Species: Synthetic
Genetic Insert: PFL_1331
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45411 Copy
Species: Synthetic
Genetic Insert: PA_4375
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45410 Copy
Species: Callicebus moloch (Red-bellied titi)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45379 Copy
Species: Synthetic
Genetic Insert: PFL_3271
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45412 Copy
Species: Macaca mulatta (Rhesus monkey)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45372 Copy
Species: Mus musculus
Genetic Insert: PKA regulatory subunit RI beta tagged by mEGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45526 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta
Vector Backbone Description: Backbone Marker:Karel Svoboda; Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, with chicken beta actin promoter and WPRE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45529 Copy
Species: Synthetic
Genetic Insert: Di-III_14_IF3_14
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23135467
Comments: Di-III_14:
mgLTRTITSQNKEELLEIALKFISQGLDLEVEFDSTDDKEIEEFERDMEDLAKKTGVQIQKQWQGNKLRIRLKGslehhhhhh
Proper citation: RRID:Addgene_45486 Copy
Species: Synthetic
Genetic Insert: Di-II_10_R2x2_769
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23135467
Comments: Di-II_10:
mLEYVIILGGSKKLIEEVKKLAENKGMEIRTVTSKDELKDLLKEFKEKSQNLFVIIIVRDEELLKKAEELAKKYNLQIRTYRAQSPDKAKDYVKEYYERIgslehhhhhh
Proper citation: RRID:Addgene_45485 Copy
Species: Synthetic
Genetic Insert: Di-V_7_R3x1_7
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23135467
Comments: Di-V_7:
mgSKIIVIISSDDTTLEELARKIKDEGLEVYILLKDKDEKRLEEKIQKLKSQGFEVRKVKDDDDIDKWIDKIKKERPQLEVRKVTDEDQAKQILEDLKKKGslehhhhhh
Proper citation: RRID:Addgene_45488 Copy
Species: Synthetic
Genetic Insert: Di-IV_5_PL2x2_6
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23135467
Comments: Di-IV_5 sequence:
mGKVLLVISTDTNIISSVQERAKHNYPGREIRTATSSQDIRDIIKSMKDNGKPLVVFVNGASQNDVNEFQNEAKKEGVSYDVLKSTDPEELTQRVREFLKTAgslehhhhhh
Proper citation: RRID:Addgene_45487 Copy
Species: Mus musculus
Genetic Insert: TRPM7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO (modified); Vector Types:Mammalian Expression, Tetracycline Inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11385574
Comments: For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor.
Proper citation: RRID:Addgene_45482 Copy
Genetic Insert: Gentamycin
Vector Backbone Description: Backbone Marker:Bagdasarian Lab; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:10447882
Comments: This is a vector control for IPTG-indicible E. coli relA gene.
Proper citation: RRID:Addgene_45475 Copy
Genetic Insert: relA
Vector Backbone Description: Backbone Marker:Bagdasarian Lab; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:10447882
Comments: This is a vector control for IPTG-indicible E. coli relA gene.
Proper citation: RRID:Addgene_45474 Copy
Genetic Insert: flaA-GFP
Vector Backbone Description: Vector Backbone:pJB98; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10447882
Comments: pflaG was generated by subcloning the 0.8kb XbaI/PstI fragment encoding GFP and T7 gene 10 rbs from pGFPmut3 into pJB98. The flaA promoter including its -35, -10, and transcriptional start sites were amplified from the chromosomal DNA of L. pneumophila 02 and ligated directly upstream of GFP using BamHI/XbaI.
pJB98 was generated from pKB5 (Berger and Isberg, 1993) by deletion of ~1.5kb HpaI/EcoRI fragment containing the ptac promoter and lacIq gene.
Proper citation: RRID:Addgene_45477 Copy
Genetic Insert: flaA-GFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pTLP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:10447882
Comments: The ~1kb BamHI/SphI flaAGFP fragment from pflaG into the ~4.6kb BamHI/SphI fragment of the ColE1 replicon pTLP6.
Proper citation: RRID:Addgene_45476 Copy
Genetic Insert: endA::frt lambda pir
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:21306445
Comments: Use lambda pir strain for R6K ori replication. endA was deleted for cleaner and higher yield plasmid preps.
Proper citation: RRID:Addgene_45472 Copy
Species: Homo sapiens
Genetic Insert: PAI-RBP v2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11001948
Comments: Our lab isolated the protein based on it's ability to bind to a region of the PAI-1 (plasminogen activator inhibitor type 1) 3'UTR that we had determined to be involved in the cAMP regulation of mRNA stability. A search of the database revealed that the cDNA for the protein had been cloned by the German Genome Project and dubbed hypothetical protein. They kindly provided us with the clone, which we subcloned into pET-15b for expression of the protein and then into CMV-Flag for mammalian expression.
Subsequently, we discovered isoforms of the protein with or without 6 and/or 15 amino acid insertions. Variant 2 has the 6 amino acid insertion but lacks the 15 amino acid insertion.
Additional reference: Heaton, JH et al 2003 Thromb Haemost 89; 959-966.
Proper citation: RRID:Addgene_45505 Copy
Genetic Insert: Src (YF)-uniRapR-cerulean
Vector Backbone Description: Backbone Size:9773; Vector Backbone:pT2KHspGGFF (Asakawa K, Kawakami K. Dev Growth Differ. 2008); Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569285
Comments: Please note that Addgene's sequencing results differ from the full plasmid sequence information from the depositing laboratory at bp# 3150, 3188, 3193, 3199, 3211, 3227, 3307, 5577 & 5578. These differences are not a concern for the function of the plasmid according to the depositing laboratory.
Proper citation: RRID:Addgene_45509 Copy
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