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Species: Synthetic
Genetic Insert: pac-T2A-FLPo
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124835 Copy
Species: Synthetic
Genetic Insert: pac-T2A-dre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124833 Copy
Species: Homo sapiens
Genetic Insert: VWF with cyan fluorescent protein fusion between D'D3 and A1 domains
Vector Backbone Description: Vector Backbone:pCS-CG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936056
Comments: I471V mutation in VWF has no impact on function
Proper citation: RRID:Addgene_124827 Copy
Species: Homo sapiens
Genetic Insert: VWF without A1 domain and D1498A mutation A2 domain
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936056
Comments: I471V mutation in VWF has no impact on function
Proper citation: RRID:Addgene_124825 Copy
Species: Homo sapiens
Genetic Insert: VWF without A1 domain
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936056
Comments: I471V mutation in VWF has no impact on function
Proper citation: RRID:Addgene_124823 Copy
Genetic Insert: VJQ Nterm
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pet29 b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_12483 Copy
Genetic Insert: AYE Nterm
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pet29 b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12948494
Comments: A synthetic gene which placed the sequence under the control of the T7 promoter, a C-terminal 6X His tag, and codon usage optimized for Escherichia coli was obtained from BlueHeron Biotechnologies. The gene was then subcloned into the pet29 b(+) vector from Novagen.
Proper citation: RRID:Addgene_12484 Copy
Genetic Insert: ssCFr
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pet29 b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_12481 Copy
Genetic Insert: luc
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11509180
Proper citation: RRID:Addgene_124892 Copy
Genetic Insert: RLuc
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11509180
Proper citation: RRID:Addgene_124893 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31069270
Proper citation: RRID:Addgene_124890 Copy
Species: Other
Genetic Insert: Cas12a
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31069270
Proper citation: RRID:Addgene_124891 Copy
Vector Backbone Description: Vector Backbone:pZHY988; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30928637
Comments: Plant codon optimized,R1335V/L1111R/D1135V/G1218R/E1219F/A1322R/T1337R
Proper citation: RRID:Addgene_124895 Copy
Species: Rattus norvegicus
Genetic Insert: rat IgG2a Hinge-G4S-Sortag-Histag
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3956; Vector Backbone:pCR4 TOPO TA; Vector Types:Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124810 Copy
Species: Homo sapiens
Genetic Insert: AR LBD
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:pSP72; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12888538
Proper citation: RRID:Addgene_12492 Copy
Species: Bacteria
Genetic Insert: iNicSnFR3b-(V7)
Vector Backbone Description: Backbone Marker:Janelia; Backbone Size:2827; Vector Backbone:pHHM.X513; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30718376
Comments: Vector was received from Janelia HHMI
Proper citation: RRID:Addgene_124881 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene#50469; Vector Backbone:pAAV-CaMKII-EGFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32025031
Comments: bioRxiv pre-print 10.1101/579250
Proper citation: RRID:Addgene_124882 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pMCSV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969085
Comments: Guide RNA to be cloned into BbsI site. Supplied modified golden-gate cloning method is required given that extra BbsI site is located within the 2A sequence.
To design guide RNAs:
-add ACCG to 5' end of positive strand (positive strand defined as strand which will contact PAM sequence at 3' end)
-add AAAC to 5' end of negative strand
Proper citation: RRID:Addgene_124889 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7162; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124887 Copy
Species: Homo sapiens
Genetic Insert: MLST8
Vector Backbone Description: Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124915 Copy
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