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Species: Ecoli
Genetic Insert: AcrAB
Vector Backbone Description: Backbone Size:6826; Vector Backbone:pBbA5k; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21556065
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_45361 Copy
Species: Pan troglodytes (chimpanzee)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45362 Copy
Genetic Insert: DHFR Ub Lek Ura3
Vector Backbone Description: Vector Backbone:pBAM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18566452
Comments: UPR pBAL Ura3 DHFR Ub Leu ek Ura3
Proper citation: RRID:Addgene_45353 Copy
Genetic Insert: DHFR Ub Mek Ura3
Vector Backbone Description: Vector Backbone:pBAM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18566452
Proper citation: RRID:Addgene_45355 Copy
Species: Synthetic
Genetic Insert: AmCyan
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4729; Vector Backbone:pmR-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27656125
Comments: the nAmCyan and the mCherry genes are separated by a P2A sequence that results in 2 separate proteins. It also includes BsmBI restriction sites flanking the nAmCyan gene which result in BsiWI and BssHII overhangs for cloning in-frame with P2A-mCherry.
Proper citation: RRID:Addgene_45350 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5488; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_45346 Copy
Vector Backbone Description: Vector Backbone:pGRB2.0; Vector Types:C. glabrata; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45341 Copy
Vector Backbone Description: Backbone Size:4910; Vector Backbone:pRS406; Vector Types:C. glabrata; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45340 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Vector Backbone:pBM16; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45339 Copy
Vector Backbone Description: Backbone Size:6134; Vector Backbone:pBM16.1; Vector Types:C. glabrata; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45334 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Vector Backbone:pBM16; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45335 Copy
Vector Backbone Description: Backbone Size:6003; Vector Backbone:pBM16.1; Vector Types:C. glabrata; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45338 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Vector Backbone:pGRB2.3; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45337 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45290 Copy
Species: Homo sapiens
Genetic Insert: TLOC1
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45293 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Vector Backbone:pBM16; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45330 Copy
Vector Backbone Description: Backbone Size:6972; Vector Backbone:pBM16; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45329 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Vector Backbone:pGRB2.3; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45328 Copy
Species: Homo sapiens
Genetic Insert: TLOC1
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Comments: The initial pDONR223 clone was obtained from the CCSB Human Orfeome Collection. Upon sequencing it was found that the construct contained a 660 bp long variant as compared to the expected 1200 bp long version, which initially had been described.
To determine if whether this shorter version is expressed in cancer cells, and exclude the possibility of this being a cloning artifact, we cloned TLOC1 from T47D cells and found both forms to be expressed. We re-cloned both the full length and shorter splice variants by ligation of BP flanked PCR products into pDONR223 by a BP Gateway reaction.
TLOC1 was transferred into DEST vectors from TLOC1 containing pDONR223 vectors through a Gateway LR reaction.
Proper citation: RRID:Addgene_45288 Copy
Vector Backbone Description: Backbone Size:5839; Vector Backbone:pBM16.1; Vector Types:C. glabrata; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Comments: Please note that Addgene's sequencing results found a single nucleotide insertion between bp# 427 and 428 when compared to the full plasmid sequence. According to the depositing laboratory, this insertion has no effect on the function of the plasmid.
Proper citation: RRID:Addgene_45321 Copy
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