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Species: Homo sapiens
Genetic Insert: TCF-4 binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10749138
Comments: TCF-4 mutant binding sequence - CCTTTGGC
Proper citation: RRID:Addgene_16559 Copy
Species: Other
Genetic Insert: T7 DNA Polymerase (unmodified)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA Polymerase that catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3'-5' exonuclease. The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165534 Copy
Species: Homo sapiens
Genetic Insert: MADMYC
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: MADMYC: the amino
terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional
repression domain (aa 1-38, the Sin3-interaction region) of Mad.
Proper citation: RRID:Addgene_16557 Copy
Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10490837
Comments: RKO (colorectal cancer cell line) mutation: 4 base-pair deletion (AGGCAGG to AGG) within the sequence corresponding to helix 3 of the CDX2 homeodomain.
Proper citation: RRID:Addgene_16554 Copy
Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10490837
Proper citation: RRID:Addgene_16553 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9659898
Proper citation: RRID:Addgene_16552 Copy
Species: Other
Genetic Insert: M.SfiI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by SfiI.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165538 Copy
Species: Viral
Genetic Insert: _C31 attB
Vector Backbone Description: Backbone Size:2171; Vector Backbone:pColE1_Omega1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34525356
Comments: attB docking site
Proper citation: RRID:Addgene_165843 Copy
Species: E. Coli
Genetic Insert: ble
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165840 Copy
Species: Viral
Genetic Insert: SR39TK
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165841 Copy
Species: Synthetic
Genetic Insert: CP6 Promoter
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Bacterial Promoter
Proper citation: RRID:Addgene_165794 Copy
Species: Synthetic
Genetic Insert: CP6 promoter
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Bacterial Promoter
Proper citation: RRID:Addgene_165795 Copy
Species: Drosophila melanogaster
Genetic Insert: R70B04
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165792 Copy
Species: Viral
Genetic Insert: SV40L
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Terminator
Proper citation: RRID:Addgene_165798 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165831 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS-TL1
Proper citation: RRID:Addgene_165832 Copy
Species: Synthetic
Genetic Insert: Promoterless-2A
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Linker - Promoter Mimic
Proper citation: RRID:Addgene_165790 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS-TL5
Proper citation: RRID:Addgene_165835 Copy
Species: E. Coli
Genetic Insert: nptII
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165836 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS-TL1
Proper citation: RRID:Addgene_165834 Copy
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