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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pOpen-T3gene1 Resource Report Resource Website |
RRID:Addgene_165576 | T3 RNA Polymerase | Other | Ampicillin | DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:43 | 0 | ||
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pOpen-XbaIM Resource Report Resource Website |
RRID:Addgene_165577 | M.XbaI | Other | Ampicillin | Site-specific DNA methyltransferase; protects DNA from cleavage by XbaI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:43 | 0 | ||
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pOpen-T4 PNK Resource Report Resource Website |
RRID:Addgene_165520 | T4 PNK | Other | Ampicillin | The most commonly used polynucleotide kinase. Used for: 5' phosphorylation of DNA/RNA for subsequent ligation; end labeling DNA or RNA for probes and DNA sequencing; removal of 3' phosphoryl groups. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-Tlipol (exo-) Resource Report Resource Website |
RRID:Addgene_165523 | Tli DNA polymerase (exo-) | Other | Ampicillin | DNA polymerase; lacks 3' to 5' exonuclease activity. Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the 'homing site' in gene that lack the intein. Comparable to VentR (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-lambda red operon Resource Report Resource Website |
RRID:Addgene_165521 | lambda red operon | Other | Ampicillin | Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA (including E. coli genome), electroporate a linear donor DNA substrate (either dsDNA or ssDNA) into E. coli expressing the lambda red enzymes. These enzymes then catalyze the homologous recombination of the substrate with the target DNA sequence (mutagenesis occurs in vivo). ~50 nucleotides of homology are required to the target site for recombination. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pIVEX2.3 TEM-5 Resource Report Resource Website |
RRID:Addgene_16561 | TEM5 | Homo sapiens | Ampicillin | PMID:11559528 | Backbone Marker:Boehringer; Backbone Size:3553; Vector Backbone:pIVEX2.3-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Extracellular region | 2026-08-08 01:11:43 | 0 | |
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pGEX TEM-1 Resource Report Resource Website |
RRID:Addgene_16560 | TEM1 | Homo sapiens | Ampicillin | PMID:11559528 | Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Extracellular region | 2026-08-08 01:11:43 | 0 | |
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pOpen-Eco31IB Resource Report Resource Website |
RRID:Addgene_165527 | Eco31IB | Other | Ampicillin | Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-SfiIR (cDNA) Resource Report Resource Website |
RRID:Addgene_165528 | SfiI (used to create cDNA libraries) | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-Pfulig Resource Report Resource Website |
RRID:Addgene_165525 | Pfu DNA Ligase | Other | Ampicillin | DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Catalyzes template-dependent ligation at temperatures of about 30-80 degrees C, and substantially retains its catalytic activity when subjected to temperatures of about 85 degrees C to about 100 degrees C. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-THEAQpolA Resource Report Resource Website |
RRID:Addgene_165512 | Taq DNA Polymerase | Other | Ampicillin | DNA polymerase used in (routine) PCR method because of its high thermostability, standard testing, screening and output testing. Has a relatively high error rate because it lacks 3'-5' exonuclease activity and proofreading functionality. 5'-3' flap endonuclease degrades displaced strand. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/ and https://freegenes.github.io/genes/BBF10K_003257.html. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-K12polLF Resource Report Resource Website |
RRID:Addgene_165511 | DNA Polymerase I, Large (Klenow) Fragment | Other | Ampicillin | DNA polymerase fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity for removal of precoding nucleotides and proofreading, but loses 5'-3' exonuclease activity. Usable for synthesis of double-stranded DNA from single-stranded templates, filling in of receded 3' ends of DNA fragments to make 5' overhang blunt, digesting away protruding 3' overhangs, preparation of radioactive DNA probes. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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px330 RAD21 gRNA Resource Report Resource Website |
RRID:Addgene_165592 | RAD21 | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-08 01:11:43 | 0 | |
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px330 p300 gRNA Resource Report Resource Website |
RRID:Addgene_165591 | P300 | Mus musculus | Ampicillin | PMID:33334824 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | NA | 2026-08-08 01:11:43 | 0 | |
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pOpen-EcoRIM Resource Report Resource Website |
RRID:Addgene_165515 | M.EcoRI | Other | Ampicillin | Methylase that recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-NotIM Resource Report Resource Website |
RRID:Addgene_165518 | M.NotI | Other | Ampicillin | Site-specific DNA methyltransferase; protects DNA from cleavage by NotI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-MMLV_RT (mut H) Resource Report Resource Website |
RRID:Addgene_165546 | Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations) | Other | Ampicillin | Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-ETSSB Resource Report Resource Website |
RRID:Addgene_165549 | Extreme Thermostable Single-Stranded DNA Binding Protein | Other | Ampicillin | ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificity of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-EcoRV Resource Report Resource Website |
RRID:Addgene_165548 | EcoRV | Other | Ampicillin | Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 | ||
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pOpen-UDG Resource Report Resource Website |
RRID:Addgene_165531 | UDG | Other | Ampicillin | Used in PreCR mix from NEB, Uracil DNA glycosylase; Monofunctional DNA glycosylase that catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil; Active on ss and dsDNA; Heat inactivation not possible. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:11:42 | 0 |
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