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Species: Homo sapiens
Genetic Insert: H2AFB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1-CT-MYC-A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11266453
Proper citation: RRID:Addgene_45172 Copy
Species: Homo sapiens
Genetic Insert: H2Afy2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11331621
Comments: Please note that Addgene's sequencing result identified a single nucleotide mismatch at bp# 653 when compared to GenBank ID AF336304. This silent mutation does not affect the protein sequence.
Proper citation: RRID:Addgene_45171 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5739; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23110063
Comments: In Sathiamoorthy et al, there is an error in the title of one of the supplementary figures. Figure S3 should read "Construction of pSAMRNAI" and in the figure the final constructed vector is "pSAMRNAI".
Proper citation: RRID:Addgene_45174 Copy
Species: Homo sapiens
Genetic Insert: H2AFB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6141; Vector Backbone:pcDNA3.1-CT-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11266453
Proper citation: RRID:Addgene_45173 Copy
Species: Homo sapiens
Genetic Insert: H2Afy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1-CT-MYC-HIS-A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11331621
Proper citation: RRID:Addgene_45166 Copy
Species: Homo sapiens
Genetic Insert: TLOC1
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Comments: The initial pDONR223 clone was obtained from the CCSB Human Orfeome Collection. Upon sequencing it was found that the construct contained a 660 bp long variant as compared to the expected 1200 bp long version.
To determine whether this shorter version is expressed in cancer cells, and to exclude the possibility of this being a cloning artifact, we cloned TLOC1 from T47D cells and found both forms to be expressed. We re-cloned both the full length and shorter splice variants by ligation of BP flanked PCR products into pDONR223 by a Gateway BP reaction.
TLOC1 was transferred into DEST vectors from TLOC1 containing pDONR223 vectors through a Gateway LR reaction.
Proper citation: RRID:Addgene_45287 Copy
Species: Homo sapiens
Genetic Insert: H2Afy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1-CT-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11331621
Proper citation: RRID:Addgene_45169 Copy
Genetic Insert: Hec1-targeted Plk1 FRET sensor
Vector Backbone Description: Backbone Size:3927; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22908307
Proper citation: RRID:Addgene_45204 Copy
Genetic Insert: Hec1-targeted mCherry Plk1 T210D
Vector Backbone Description: Backbone Size:5348; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22908307
Proper citation: RRID:Addgene_45205 Copy
Species: Homo sapiens
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9786947
Comments: The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis.
Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez F, Smith CL, Youle RJ
In Dev Cell. 2001 Oct;1(4):515-25.
Proper citation: RRID:Addgene_45161 Copy
Genetic Insert: CeFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20923671
Comments: This construct was made to create transgenic mice in which hVoS 1.5 is expressed under the thy1 promoter only in neuronal cells.
The cerulean sequence has a silent G-C mutation at 687 that differs from standard sequence.
Addgene has sequenced a portion of this plasmid which confirms part of thy-1 with some ambiguous bases in the sequencing results.
Proper citation: RRID:Addgene_45281 Copy
Species: Homo sapiens
Genetic Insert: pLAC-C25
Vector Backbone Description: Backbone Marker:New England Biolabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression, Protein Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16923831
Comments: The last 25 amino acids of lacritin were deleted in this construct (aa 114-138).
Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence. The depositing laboratory indicates that these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_45283 Copy
Species: Heteractis crispa
Genetic Insert: hcRed
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45286 Copy
Species: Homo sapiens
Genetic Insert: TNIK
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Comments: The TNIK open reading frame was PCR amplified with BP flanked primers from the vector acquired from Open Biosystems and ligated into a pDONR223 vector in a BP Gateway ligation reaction. The TNIK open reading frame was subsequently ligated into the pBabe_puro_DEST_flag backbone in a Gateway LR ligation reaction.
Proper citation: RRID:Addgene_45276 Copy
Genetic Insert: Cre Stoplight
Vector Backbone Description: Backbone Marker:Invitrogene; Backbone Size:3342; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11730010
Proper citation: RRID:Addgene_45150 Copy
Species: Homo sapiens
Genetic Insert: LRRC31
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45271 Copy
Species: Homo sapiens
Genetic Insert: MCOPS3
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45270 Copy
Species: Homo sapiens
Genetic Insert: TLOC1
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45273 Copy
Species: Homo sapiens
Genetic Insert: GPR160
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45274 Copy
Species: Synthetic
Genetic Insert: E-coli-pathways-QconCAT16
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:5211; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25205128
Proper citation: RRID:Addgene_45144 Copy
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