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http://www.addgene.org/165601

Species: Homo sapiens
Genetic Insert: ribosomal protein S6 kinase A5
Vector Backbone Description: Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33563994

Proper citation: RRID:Addgene_165601 Copy   

•   Source: Addgene

http://www.addgene.org/165565

Species: Other
Genetic Insert: RNAse A
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA. Effectively cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide which forms a 2',3'-cyclic phosphate which is then hydrolyzed to the corresponding 3'-nucleoside phosphate. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165565 Copy   

•   Source: Addgene

http://www.addgene.org/165566

Species: Other
Genetic Insert: Thermus thermophilus (Tth) RT
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165566 Copy   

•   Source: Addgene

http://www.addgene.org/165560

Species: Other
Genetic Insert: T4 gene 32
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165560 Copy   

•   Source: Addgene

http://www.addgene.org/165604

Genetic Insert: hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
Vector Backbone Description: Vector Backbone:pGHUC_R25; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33441553

Proper citation: RRID:Addgene_165604 Copy   

•   Source: Addgene

http://www.addgene.org/165605

Genetic Insert: hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab (Addgene #165604); Vector Backbone:pGG184; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33441553

Proper citation: RRID:Addgene_165605 Copy   

•   Source: Addgene

http://www.addgene.org/165569

Species: Other
Genetic Insert: Tth DNA Ligase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: High fidelity version Taq DNA Ligase. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165569 Copy   

•   Source: Addgene

http://www.addgene.org/165606

Genetic Insert: ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pB1H2_UV2-ω-dCas9::K2_UV5-sgRNA; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33441553

Proper citation: RRID:Addgene_165606 Copy   

•   Source: Addgene

http://www.addgene.org/165550

Species: Other
Genetic Insert: T4 PGD (T4 Endonuclease V)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases cis-syn cyclobutane pyrimidine dimers, including T^T, T^C and C^C, generating an AP site. The AP lyase activity cleaves an AP site via beta-elimination, creating a 1 nucleotide DNA gap with 3'-alpha, beta-unsaturated aldehyde and 5'-phosphate termini. Also known as T4 Endonuclease V. This enzyme in combination with other enzymes from E. coli (UDG, fpg, Endonuclease IV, Endonuclease VIII), Bst-DNA pol and Taq/Tth-DNA Ligase forms the proprietary NEB Enzyme mix preCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165550 Copy   

•   Source: Addgene

http://www.addgene.org/165551

Species: Other
Genetic Insert: Endonuclease IV E. coli
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Used in the preCR mix from NEB; DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165551 Copy   

•   Source: Addgene

http://www.addgene.org/165556

Species: Other
Genetic Insert: Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165556 Copy   

•   Source: Addgene

http://www.addgene.org/165558

Species: Other
Genetic Insert: M.EcoRV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by EcoRV. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165558 Copy   

•   Source: Addgene

http://www.addgene.org/165585

Species: Mus musculus
Genetic Insert: BRG1
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824

Proper citation: RRID:Addgene_165585 Copy   

•   Source: Addgene

http://www.addgene.org/165586

Species: Mus musculus
Genetic Insert: BRM
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824

Proper citation: RRID:Addgene_165586 Copy   

•   Source: Addgene

http://www.addgene.org/165589

Species: Mus musculus
Genetic Insert: MAU2
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824

Proper citation: RRID:Addgene_165589 Copy   

•   Source: Addgene

http://www.addgene.org/165587

Species: Mus musculus
Genetic Insert: CBP
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824

Proper citation: RRID:Addgene_165587 Copy   

•   Source: Addgene

http://www.addgene.org/165588

Species: Mus musculus
Genetic Insert: CTCF
Vector Backbone Description: Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33334824

Proper citation: RRID:Addgene_165588 Copy   

•   Source: Addgene

http://www.addgene.org/165575

Species: Other
Genetic Insert: RNAse H
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus. Along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis, and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA pre-primer. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165575 Copy   

•   Source: Addgene

http://www.addgene.org/165572

Species: Other
Genetic Insert: Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165572 Copy   

•   Source: Addgene

http://www.addgene.org/165612

Genetic Insert: SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
Vector Backbone Description: Backbone Marker:Church Lab (Addgene #43803); Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33441553
Comments: This plasmid was constructed by Dr. Agmon in the Boeke lab and first described in: Agmon, N. et al. Phylogenetic debugging of a complete human biosynthetic pathway transplanted into yeast. Nucleic Acids Res. 48, 486–499 (2020)

Proper citation: RRID:Addgene_165612 Copy   

•   Source: Addgene