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Genetic Insert: CMVe-betaAc-STOP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMVe-betaAc-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11410679
Comments: CMV-enhancer-betaAc driven luciferase reporter gene containing a floxed stop cassette.
Proper citation: RRID:Addgene_12463 Copy
Species: Homo sapiens
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:0; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16122702
Comments: EcoRV site was destroyed during cloning and replaced with a new NotI site.
Proper citation: RRID:Addgene_12461 Copy
Species: Homo sapiens
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16122702
Proper citation: RRID:Addgene_12460 Copy
Species: Synthetic
Genetic Insert: CellTagB
Vector Backbone Description: Backbone Size:8426; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31072405
Comments: The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014).
Please visit https://www.biorxiv.org/content/10.1101/335547v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_124592 Copy
Species: Synthetic
Genetic Insert: CellTagA
Vector Backbone Description: Backbone Size:8426; Vector Backbone:pSMAL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31072405
Comments: The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014).
Please visit https://www.biorxiv.org/content/10.1101/335547v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_124591 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Marker:https://www.addgene.org/19407/; Backbone Size:4200; Vector Backbone:pTretight-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961879
Comments: Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124506 Copy
Species: Mus musculus
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Marker:Novogen; Backbone Size:5369; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124627 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124583 Copy
Species: Synthetic
Genetic Insert: CggR promoter element
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124582 Copy
Species: Synthetic
Genetic Insert: cggR_R250A
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124585 Copy
Species: Synthetic
Genetic Insert: cggR
Vector Backbone Description: Vector Backbone:pHO - plasmid; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31885198
Comments: Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124584 Copy
Vector Backbone Description: Backbone Marker:PMID: 15474295; Backbone Size:7509; Vector Backbone:pSFS2A; Vector Types:Synthetic Biology, Candida albicans expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30747494
Comments: Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The different orientation of the F1 ori does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result.
Proper citation: RRID:Addgene_124587 Copy
Species: Homo sapiens
Genetic Insert: GluR2_adRNA(2,100,50)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Proper citation: RRID:Addgene_124622 Copy
Species: Homo sapiens
Genetic Insert: ADAR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Comments: Also expresses 2 copies of GluR2_adRNA(1,20,6) from human and mouse U6 promoters
Proper citation: RRID:Addgene_124620 Copy
Species: Homo sapiens
Genetic Insert: MCP_ADAR1 DD (E1008Q)_NLS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Comments: Also expresses MS2_adRNA targeting the RAB7A transcript
Proper citation: RRID:Addgene_124626 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31961879
Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector).
To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted.
The mTurquoise2 tagged variant was generated by exchanging an AgeI/BsrgI sGFP2 fragment for an AgeI/BsrgI mTurquoise fragment. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124504 Copy
Species: Homo sapiens
Genetic Insert: MS2_adRNA_RAB7A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Proper citation: RRID:Addgene_124624 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124691 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving EGFP under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124692 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Backbone Marker:Addgene: 85866; Vector Backbone:AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30778069
Comments: Bi-directional expression vector driving PD-L1 under control of a miR-17 MRE with NGFR as an internal control
Proper citation: RRID:Addgene_124684 Copy
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