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Species: Aequorea victoria
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Ezaz-Nikpay et al, 1994 Protein Science; Backbone Size:3531; Vector Backbone:pKEN; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: An XbaI site was introduced at the beginning of GFP, see attached map for more details.
The protein is expressed from IPTG inducible promoter in pKEN vector. GFP is cloned as XbaI-PstI fragment into the polylinker; there is also NdeI site at the AUG that can be used.
Mut 2 is probably best for expression in bacteria.
Overexpression of GFP renders the cells osmosensitive. In the absence of IPTG, DH12 carrying the enclosed plasmids will lyse in LB. We recommend growing this plasmid in low salt LB (<5g/L NaCl); alternatively use E.coli strain that grossly overproduces lacI and totally shuts expression down.
Proper citation: RRID:Addgene_20409 Copy
Species: Rattus norvegicus
Genetic Insert: AnxA6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: AnxA6 was cut out of pGEMT with HindIII and XbaI and ligated into pcDNA3.1 + with HindIII and XbaI
Proper citation: RRID:Addgene_20407 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS8
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20247 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO-conjugating enzyme Ubc9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20368 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS7
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20246 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS5
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20244 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin B +1 transcript
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20254 Copy
Species: Homo sapiens
Genetic Insert: TopBP1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His.
NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab.
Proper citation: RRID:Addgene_20375 Copy
Species: human immunodeficiency virus type 1
Genetic Insert: HIV-1 protease
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20253 Copy
Species: Homo sapiens
Genetic Insert: TopBP1 (1-1013)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20374 Copy
Species: Homo sapiens
Genetic Insert: SIRT1
Vector Backbone Description: Backbone Size:6470; Vector Backbone:pMIR-REPORT; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: There are mismatches between Addgene's sequence and NCBI sequence for SIRT1 UTR. Deposit is aware of these changes and doesn't think they affect function.
Proper citation: RRID:Addgene_20379 Copy
Species: Homo sapiens
Genetic Insert: IKBKE
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20504 Copy
Species: Homo sapiens
Genetic Insert: MOBKL1A
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20503 Copy
Species: Homo sapiens
Genetic Insert: HK2
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20501 Copy
Species: Homo sapiens
Genetic Insert: ITPK1
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20508 Copy
Species: Homo sapiens
Genetic Insert: ITK
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20507 Copy
Species: Homo sapiens
Genetic Insert: CLK1
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20463 Copy
Species: Homo sapiens
Genetic Insert: CKS2
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Very short isoform, only 79aa long.
Proper citation: RRID:Addgene_20462 Copy
Species: Homo sapiens
Genetic Insert: CSNK1A1L
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20467 Copy
Species: Homo sapiens
Genetic Insert: CLK3
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20465 Copy
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