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Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 2 parts are promoters in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65116 Copy
Species: Synthetic
Genetic Insert: ConL1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 1 parts are 5' assembly connectors in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65110 Copy
Species: Synthetic
Genetic Insert: ConL3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 1 parts are 5' assembly connectors in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65112 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pRNR1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 2 parts are promoters in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65128 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pRPL18B
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 2 parts are promoters in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65124 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pdCas9-Marraffini (pACYC184); Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_65006 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pPAB1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 2 parts are promoters in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pHHF1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 2 parts are promoters in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65122 Copy
Species: Synthetic
Genetic Insert: 6XHis_3XFlag
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein purification tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65147 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubi-Y
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein degradation tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65149 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubi-M
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein degradation tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65148 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal fluorescent protein tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65145 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubi-R
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein degradation tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65150 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are used for coding sequences. This plasmid encodes for a fluorescent protein. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65151 Copy
Species: Synthetic
Genetic Insert: sgRNA Dropout
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 234 parts substitute a concatenation of part types 2, 3 and 4. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65157 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tSSA1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65159 Copy
Species: Synthetic
Genetic Insert: GFP dropout
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 234r is used for the construction of dropout vectors. This plasmid encodes for a fluorescent dropout. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65154 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 3b parts are used for coding sequences. This plasmid encodes for a fluorescent protein. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65153 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tADH1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65160 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tENO2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Type 4 parts are terminators in the Dueber YTK For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65162 Copy
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