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Species: Homo sapiens
Genetic Insert: Gal4-TET1-878-911(T898A mutation)
Vector Backbone Description: Backbone Marker:University of Leicester; Vector Backbone:pLeicester 84; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279502
Proper citation: RRID:Addgene_124403 Copy
Species: Synthetic
Genetic Insert: SpyDock
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3641; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30988307
Proper citation: RRID:Addgene_124618 Copy
Species: Homo sapiens
Genetic Insert: GluR2_adRNA(1,20,6)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30737497
Comments: A second copy of the GluR2_adRNA_(1,20,6) is expressed from a mouse U6 promoter
Proper citation: RRID:Addgene_124619 Copy
Species: Homo sapiens
Genetic Insert: PU.1 ETS domain (residues 167-272)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30295801
Proper citation: RRID:Addgene_124616 Copy
Species: Triticum aestivum
Genetic Insert: TaNAM-A1
Vector Backbone Description: Backbone Size:15926; Vector Backbone:pGWB21; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31533618
Comments: Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124572 Copy
Species: Triticum aestivum
Genetic Insert: TaNAM-A1
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2817; Vector Backbone:pCR8; Vector Types:TOPO Gateway Cloning Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31533618
Comments: Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124574 Copy
Species: Triticum turgidum ssp. dicoccoides
Genetic Insert: TaNAM-B1
Vector Backbone Description: Backbone Size:15622; Vector Backbone:pGWB12; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31533618
Comments: Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124573 Copy
Species: Triticum turgidum ssp. dicoccoides
Genetic Insert: TtNAM-B1 (1-215)
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2817; Vector Backbone:pCR8; Vector Types:TOPO Gateway Cloning Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31533618
Comments: Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124577 Copy
Genetic Insert: TCF/LEF binding sites
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4871; Vector Backbone:pTA-Luc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites.
This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 7 TCF/LEF binding sites were cloned into the Mlu1 site of this vector (7 copies of: AGATCAAAGGgggta, with TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the TCF/LEF site).
Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites.
Proper citation: RRID:Addgene_12456 Copy
Species: Homo sapiens
Genetic Insert: NPM1 Homology Arms with linker-mTagRFP-T
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the C-terminus of human NPM1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: KPNSAVDGTAGPGSIAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_124608 Copy
Species: Mus musculus
Genetic Insert: UBP43
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11788588
Proper citation: RRID:Addgene_12458 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Size:4679; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16352599
Proper citation: RRID:Addgene_12453 Copy
Species: Homo sapiens
Genetic Insert: EFP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16352599
Comments: Digestion by KpnI and XhoI should give a 5.4 kb fragment and a 2.4 kb fragment.
Compared to GenBank ID NP_005073.2, the EFP insert in this plasmid appears to contain a P385L mutation, whereas when compared to GenBank ID AB384815.1 the EFP insert appears to contain G89V and A185T mutations. These apparent mutations may represent valid polymorphisms and reflect the sequence of the original human cDNA clone. The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_12452 Copy
Species: Mus musculus
Genetic Insert: UBP43
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16710296
Proper citation: RRID:Addgene_12454 Copy
Species: Mus musculus
Genetic Insert: Soma-localized ChrimsonR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842660
Proper citation: RRID:Addgene_124603 Copy
Species: E. coli
Genetic Insert: Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
Vector Backbone Description: Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31036827
Comments: Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_124601 Copy
Species: Homo sapiens
Genetic Insert: WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: wild-type tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. Wild-type tRNA has high pol-III promoter activity with high processing. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124541 Copy
Species: Homo sapiens
Genetic Insert: delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124540 Copy
Species: Homo sapiens
Genetic Insert: deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124543 Copy
Species: Homo sapiens
Genetic Insert: delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
Vector Backbone Description: Vector Backbone:P1-EYFP-pA (Construct 5) (Addgene: 55197); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30940799
Comments: Engineered tRNA scaffold-flanked sgRNA loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.
Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_124545 Copy
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