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Species: Other
Genetic Insert: gal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33594068
Proper citation: RRID:Addgene_165068 Copy
Species: Homo sapiens
Genetic Insert: APC
Vector Backbone Description: Backbone Marker:Vogelstein lab (Addgene plasmid# 16440); Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Proper citation: RRID:Addgene_16507 Copy
Species: Other
Genetic Insert: gal80Arm1-P-TPI1-KanMX4-gal80Arm2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33594068
Proper citation: RRID:Addgene_165069 Copy
Species: Homo sapiens
Genetic Insert: Pig10
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4500; Vector Backbone:pBK-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9305847
Proper citation: RRID:Addgene_16504 Copy
Species: Other
Genetic Insert: T-RPL3>ERG20>P-GAL1-P-GAL2>Y-FAST>ErB1.2A-AcNES1>T-RPL41B
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33594068
Comments: Please note Addgene NGS result identified several mutations in LEU2- V73A, A82G, L199V, D304N. Depositor confirms this does not affect plasmid function. On the contrary the depositor has found "significant improvement on the productivities in the strain harbouring this plasmid."
Proper citation: RRID:Addgene_165067 Copy
Species: Homo sapiens
Genetic Insert: Pig9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9305847
Proper citation: RRID:Addgene_16503 Copy
Genetic Insert: Mad-binding element from Vg
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9660945
Comments: Four copies of the Mad-binding element from Vg (GCTTGGACTGCCGTCGCGATTC) are cloned into pGL3.
Proper citation: RRID:Addgene_16501 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9660945
Comments: Two copies of the Smad binding site (taaGTCTAGACggcaGTCTAGACgtac) are cloned into pGL3.
Proper citation: RRID:Addgene_16500 Copy
Species: Other
Genetic Insert: P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33594068
Proper citation: RRID:Addgene_165061 Copy
Species: Other
Genetic Insert: P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33594068
Proper citation: RRID:Addgene_165064 Copy
Species: Other
Genetic Insert: T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33594068
Proper citation: RRID:Addgene_165062 Copy
Species: Homo sapiens
Genetic Insert: TRB
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165002 Copy
Species: Homo sapiens
Genetic Insert: TRAV
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165001 Copy
Species: Homo sapiens
Genetic Insert: TRB
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165006 Copy
Species: Homo sapiens
Genetic Insert: TRAV
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165007 Copy
Species: Homo sapiens
Genetic Insert: TRB
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165004 Copy
Species: Homo sapiens
Genetic Insert: TRAV
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165005 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Vector Backbone:pMaster; Vector Types:Mammalian Expression, Episomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088009
Proper citation: RRID:Addgene_165080 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Vector Backbone:pMaster; Vector Types:Mammalian Expression, Episomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088009
Comments: Please note that Addgene's NGS found that the bGH poly(A) signal between one of the oris and Lin28 is absent as compared to the annotated sequence provided by the depositor.
Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.
Proper citation: RRID:Addgene_165081 Copy
Species: Homo sapiens
Genetic Insert: TRB
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_165008 Copy
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