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Species: Synthetic
Genetic Insert: AtCRU3 T494 TALENs
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: pZHY013 reference:
Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44464 Copy
Genetic Insert: A luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutation
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pGreenII 0579-1 (35S:: LUC); Vector Types:Luciferase, Plant Transformation; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_44466 Copy
Genetic Insert: NOR-gate
Vector Backbone Description: Vector Backbone:pSB4A5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44452 Copy
Genetic Insert: OR-gate
Vector Backbone Description: Vector Backbone:pSB4A5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44451 Copy
Genetic Insert: XNOR-gate
Vector Backbone Description: Vector Backbone:pSB4A5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44454 Copy
Genetic Insert: XOR-gate
Vector Backbone Description: Vector Backbone:pSB4A5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44453 Copy
Genetic Insert: pTEt-Bxb1, pBAD-TP901
Vector Backbone Description: Vector Backbone:J64100; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44456 Copy
Genetic Insert: XNOR-gate
Vector Backbone Description: Vector Backbone:pSB4A5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44455 Copy
Genetic Insert: AND-gate
Vector Backbone Description: Vector Backbone:pSB4A5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23539178
Proper citation: RRID:Addgene_44449 Copy
Vector Backbone Description: Vector Backbone:pHC115; Vector Types:Bacterial Expression, RK2, pMB1; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23648175
Proper citation: RRID:Addgene_44448 Copy
Species: Synthetic
Genetic Insert: Horseradish peroxidase fused to the transmembrane domain of PDGFR
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23371551
Comments: This construct has the Ig k-chain leader sequence on the N-terminus, which directs the protein to the secretory pathway. HRP faces the extracellular side of the plasma membrane.
Proper citation: RRID:Addgene_44441 Copy
Vector Backbone Description: Vector Backbone:pHC115; Vector Types:Bacterial Expression, RK2, pMB1; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23648175
Proper citation: RRID:Addgene_44447 Copy
Species: Homo sapiens
Genetic Insert: TFEB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22692423
Proper citation: RRID:Addgene_44446 Copy
Species: Synthetic
Genetic Insert: PTETREG promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4456; Vector Backbone:pRS403; Vector Types:Yeast Expression, Synthetic Biology, Expression regulator/reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22511863
Comments: This plasmids was created as follows. First, the PTETREG promoter consisting of two tetO2 sites upstream of the minimal PCYC1 promoter was amplified from the pBB247 plasmid (Becskei, Séraphin, and Serrano, EMBO 2001, PMID: 11350942), and inserted into the pDN-G1GZmh plasmid between the AflII and BamHI sites instead of the PGAL1-D12 promoter resulting in the pDN-T2dGZmh plasmid. In order to facilitate the planned integration of the reporter plasmid into the his3Δ200 locus of the YPH500 strain, a small region bearing homology to the his3Δ200 locus was constructed by PCR and inserted in front of the HIS3 gene between the AhdI and AfeI sites of the pDN-T2dGZmh plasmid, resulting in the pDN-T2dGZmlh plasmid. Next, a second ADH1 terminator was removed from the pDN-T2dGZmlh plasmid, resulting in the final reporter pDN-T2dGZmxh plasmid bearing the yEGFP::zeoR fluorescent reporter gene under the control of the PTETREG rtTA-MF inducible promoter.
Proper citation: RRID:Addgene_44552 Copy
Species: Homo sapiens
Genetic Insert: MAVS-Fis1 chimeric protein
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pMSCV2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20451243
Proper citation: RRID:Addgene_44556 Copy
Genetic Insert: hhf2
Vector Backbone Description: Vector Backbone:pUK499; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3048701
Proper citation: RRID:Addgene_44547 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HA-htz1
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16543223
Proper citation: RRID:Addgene_44549 Copy
Genetic Insert: hhf2
Vector Backbone Description: Vector Backbone:pUK499; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3048701
Proper citation: RRID:Addgene_44542 Copy
Genetic Insert: hhf2
Vector Backbone Description: Vector Backbone:pUK499; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3048701
Proper citation: RRID:Addgene_44541 Copy
Species: Danio rerio
Genetic Insert: Angptl4 Intronic Region 3.4
Vector Backbone Description: Backbone Size:5883; Vector Backbone:pDestTol2pA2; Vector Types:Tol2 vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_44662 Copy
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