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Species: A. niger
Genetic Insert: pTPI1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118075 Copy
Species: Synthetic
Genetic Insert: Fab_Theo-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119048 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119884 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126601 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126600 Copy
Vector Backbone Description: Backbone Size:5903; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126606 Copy
Species: Synthetic
Genetic Insert: hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31672972
Comments: pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134316 Copy
Species: Synthetic
Genetic Insert: CymR(AM)-mRFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149494 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149559 Copy
Species: Synthetic
Genetic Insert: sgRNAftsI
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149563 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(RBSmut); Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149580 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149573 Copy
Species: Acidaminococcus sp. BV3L6
Genetic Insert: Ascpf1
Vector Backbone Description: Vector Backbone:pSIM18; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33710766
Proper citation: RRID:Addgene_153034 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:26934495
Proper citation: RRID:Addgene_157877 Copy
Species: Other
Genetic Insert: crRNA
Vector Backbone Description: Backbone Marker:from Houhui Song's Lab; Vector Backbone:pSL001; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Mycobacteria Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28646112
Comments: Please note that several differences were identified in the plasmid backbone compared to the depositor's sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_158708 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158721 Copy
Genetic Insert: scbR
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology, Integrative into Streptomyces genome via serine integrase (like parent pSET152); Bacterial Resistance:Hygromycin
Defining Citation: PMID:29130169
Comments: Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. ‘Orthogonal Regulatory Circuits for Escherichia Coli Based on the γ-Butyrolactone System of Streptomyces Coelicolor’. ACS Synthetic Biology 7, no. 4 (2018): 1043–55. https://doi.org/10.1021/acssynbio.7b00425.
pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase.
Proper citation: RRID:Addgene_172356 Copy
Genetic Insert: pmyc1tetO
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: pmyc1tetO is a tet operator containing promoter for mycobacteria. Also see Pubmed IDs 18059281 and 15687379.
Derivative of pMS2 containing Pmyc1tetO. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17972 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Psmyc-tetR#28
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20319 Copy
Species: Homo sapiens
Genetic Insert: PIK3C3 and PIK3R4
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6321; Vector Backbone:pVitro2; Vector Types:Mammalian Expression, bicistronic; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18957027
Proper citation: RRID:Addgene_24055 Copy
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