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Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395539
Comments: The stop codon of cyclin B1 in the pCMX vector was mutated to a Mfe1 site to link cyclin B1 to MmGFP. Cyclin B1 was fused to the second amino acid residue of MmGFP by a 5 amino acid linker (AGAQF). The construct is cloned as a HindIII-BamHI fragment into pCMX.
The Cyclin insert contains a V5A polymorphism which has no functional consequence. More information on MmGFP can be found in the following reference: Zernicka-Goetz, M., Pines, J., M., Ryan, K., Siemering, K.R., Haseloff, J., and Gurdon, J.B. (1996) ‘An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
Proper citation: RRID:Addgene_26061 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11741955
Proper citation: RRID:Addgene_25976 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11741955
Proper citation: RRID:Addgene_25975 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15371409
Proper citation: RRID:Addgene_25978 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9516483
Proper citation: RRID:Addgene_25972 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9516483
Proper citation: RRID:Addgene_25971 Copy
Species: Mus musculus
Genetic Insert: L-Myc
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo; Backbone Size:6340; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660764
Proper citation: RRID:Addgene_26023 Copy
Species: Homo sapiens
Genetic Insert: L-Myc
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo; Backbone Size:6340; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660764
Proper citation: RRID:Addgene_26022 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9516483
Proper citation: RRID:Addgene_25970 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo; Backbone Size:6340; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660764
Proper citation: RRID:Addgene_26024 Copy
Genetic Insert: Tension Sensor module
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613844
Comments: The are a few discrepancies with the author sequence. THese mismatches are in the pcDNA3.1(+) part of the vector, not the specific inserts. So they should be of little consequence.
Proper citation: RRID:Addgene_26021 Copy
Species: Homo sapiens
Genetic Insert: tumor protein p53
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5385; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11034215
Comments: There is a L383H mutation in the sequence. The mutation should not affect plasmid function.
Proper citation: RRID:Addgene_25989 Copy
Species: Homo sapiens
Genetic Insert: ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2
Vector Backbone Description: Backbone Marker:James Thomson; Backbone Size:6500; Vector Backbone:pSIN-EF2-MCS-IRES-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19670287
Comments: Please note that the backbone of this plasmid is larger than the reported 6.5kb (see attached diagnostic digest).
Proper citation: RRID:Addgene_25983 Copy
Species: Rattus norvegicus
Genetic Insert: mammalian target of rapamycin-R2505P
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20190810
Proper citation: RRID:Addgene_26038 Copy
Species: Rattus norvegicus
Genetic Insert: mammalian target of rapamycin-S2215Y
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20190810
Proper citation: RRID:Addgene_26037 Copy
Species: D. Striata
Genetic Insert: cp193g7
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pBad/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20521333
Proper citation: RRID:Addgene_25950 Copy
Species: D. Striata
Genetic Insert: cp196
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pBad/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20521333
Proper citation: RRID:Addgene_25952 Copy
Species: D. Striata
Genetic Insert: cp204
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pBad/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20521333
Comments: Addgene's sequence with pBAD-F shows minor mismatches and gaps with the reference sequence from the depositing lab. These discrepancies are a result of mistakes made during sequence assembly and should not impact plasmid function.
Please note that growing the construct at 30-34C may increase protein yield (36-48hours).
Proper citation: RRID:Addgene_25951 Copy
Species: Homo sapiens
Genetic Insert: LEMD3 LEM domain containing 3
Vector Backbone Description: Backbone Size:3919; Vector Backbone:pSVK3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11896184
Proper citation: RRID:Addgene_26002 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 2
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19883615
Proper citation: RRID:Addgene_25969 Copy
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