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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pMC234r-ccdB/TPK2 Resource Report Resource Website |
RRID:Addgene_176179 | dropout cassette containing toxic genes ccdB and TPK2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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pETx-SR6v15 Resource Report Resource Website |
RRID:Addgene_176212 | SR6v15 | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:5639; Vector Backbone:pETx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 0 | ||
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pMC6-spacer Resource Report Resource Website |
RRID:Addgene_176178 | 41 bp non-coding DNA spacer from pYTK048 | Synthetic | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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pETx-SR6v15.t Resource Report Resource Website |
RRID:Addgene_176214 | SR6v15.t | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:5639; Vector Backbone:pETx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 0 | ||
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pETx-SR6v15.d Resource Report Resource Website |
RRID:Addgene_176213 | SR6v15.d | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:5639; Vector Backbone:pETx; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:04 | 0 | ||
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pAAV-hAdipoq-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_176215 | GFP | Other | Ampicillin | PMID:32184391 | Backbone Size:5050; Vector Backbone:pAAV-CBA-w; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 1 | ||
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pMC8b-XI3up Resource Report Resource Website |
RRID:Addgene_176170 | 5' homology for integration site XI-3 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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pMC8b-XI5up Resource Report Resource Website |
RRID:Addgene_176172 | 5' homology for integration site XI-5 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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DR.GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_17617 | HLA-DRa promoter | Homo sapiens | Ampicillin | PMID:11781219 | SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs). We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells. Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells. | Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 1 | |
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pMC7-XII2dw Resource Report Resource Website |
RRID:Addgene_176175 | 3' homology for integration site XII-2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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pMC8b-XII2up Resource Report Resource Website |
RRID:Addgene_176174 | 5' homology for integration site XII-2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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CAG.mCherry-INBox(AAA)-eDHFR Resource Report Resource Website |
RRID:Addgene_176207 | mCherry-INBoxAAA-eDHFR | Homo sapiens | Ampicillin | PMID:33904910 | Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:04 | 0 | ||
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pBx1-VHH template2-3XMyc-Spacer Resource Report Resource Website |
RRID:Addgene_176209 | VHH template2-3XMyc-Spacer | Synthetic | Ampicillin | PMID:34535642 | Backbone Size:2561; Vector Backbone:pBx1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 0 | ||
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pMC8b-XI1up Resource Report Resource Website |
RRID:Addgene_176166 | 5' homology for integration site XI-1 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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CAG.mCherry-eDHFR Resource Report Resource Website |
RRID:Addgene_176201 | mCherry-eDHFR | E. coli | Ampicillin | PMID:33904910 | Please note that there are minor differences between the Addgene NGS result and the depositor's sequence. These differences do not alter plasmid function. | Backbone Marker:Dr. Eugene Makeyev; Backbone Size:-1; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 0 | |
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pMC7-XI1dw Resource Report Resource Website |
RRID:Addgene_176167 | 3' homology for integration site XI-1 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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pBH31(gfcD-10xHis) Resource Report Resource Website |
RRID:Addgene_176200 | gfcD | E. coli E2348/69 O127:H6 | Ampicillin | PMID:34780538 | encodes (pelC signal sequence)-His10-GfcD(22-698) | Backbone Marker:from Dr Frank Collart; Vector Backbone:pBH31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 0 | |
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pMC7-XI2dw Resource Report Resource Website |
RRID:Addgene_176169 | 3' homology for integration site XI-2 | Saccharomyces cerevisiae | Chloramphenicol | PMID:34860007 | Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). | Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-08 01:13:03 | 0 | |
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CAG.mCherry-INBox-eDHFR Resource Report Resource Website |
RRID:Addgene_176202 | mCherry-INBox-eDHFR | Homo sapiens | Ampicillin | PMID:33904910 | Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:13:03 | 0 | ||
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EF.delmFasL.CMV.GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_17620 | delFasL | Mus musculus | Ampicillin | PMID:12788657 | The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP, with a unique EcoRV site available to insert a gene of interest under the control of the EF promoter. Noncleavable mouse delFasL cDNA was constructed using PCR cloning to delete the exon 2 region, which contains the metalloproteinase cleavage site. The delFasL gene was then cloned into the EcoRV site of EF.CMV.GFP to make the EF.delFasL-CMV.GFP LV. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. | Backbone Size:0; Vector Backbone:EF.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Noncleavable mouse delFasL . Deleted the exon 2 region, which contains the metalloproteinase cleavage site. | 2026-08-08 01:13:03 | 1 |
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