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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDisplay FLIPE-1m Resource Report Resource Website 1+ mentions |
RRID:Addgene_13548 | FLIP-E A207W | E. coli | Ampicillin | PMID:15939876 | For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html | Backbone Marker:Invitrogen; Backbone Size:5325; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | A207W in binding protein | Addgene | 2026-10-17 02:36:57 | 2 |
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pDisplay FLIPE-600n Resource Report Resource Website 1+ mentions |
RRID:Addgene_13545 | FLIP-E WT | E. coli | Ampicillin | PMID:15939876 | For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html | Backbone Marker:Invitrogen; Backbone Size:5325; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:36:56 | 6 | |
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pDisplay FLIPE-10u Resource Report Resource Website |
RRID:Addgene_13546 | FLIP-E A207R | E. coli | Ampicillin | PMID:15939876 | For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html | Backbone Marker:Invitrogen; Backbone Size:5325; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | A207R in binding protein | Addgene | 2026-10-17 02:36:57 | 0 |
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pRSET FLII275Pglu-4.6m Resource Report Resource Website |
RRID:Addgene_13565 | FLIPglu F16A G275S-ECFP-K276R | E. coli | Ampicillin | PMID:16131659 | For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html Citrine is modified version of EYFP. | Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | F16A, ECFP insert in mglB | Addgene | 2026-10-17 02:36:58 | 0 |
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pRSET FLII12Pglu-600u Resource Report Resource Website 1+ mentions |
RRID:Addgene_13563 | FLIPglu F16A Y12S-ECFP-D13R | E. coli | Ampicillin | PMID:16131659 | For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html Citrine is modified version of EYFP. | Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | F16A ECFP insertion in binding protein | Addgene | 2026-10-17 02:36:58 | 1 |
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pcDNA3.1 FLIPglu-600u Resource Report Resource Website 1+ mentions |
RRID:Addgene_13572 | FLIPglu F16A | E. coli | Ampicillin | PMID:12649277 | For more information, see http://dpbweb.stanford.edu/sites/www.dpb.ciw.edu/files/frommer/nanosensors/index.html | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | F16A in binding protein | Addgene | 2026-10-17 02:36:59 | 1 |
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pRSET FLIPglu-600uDelta11 Ares-Aphrodite Resource Report Resource Website |
RRID:Addgene_13571 | FLIPglu F16A Delta11 Aphrodite | E. coli | Ampicillin | PMID:16935985 | For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html EYFP was exchanged with Aphrodite (accession number DQ525025), which is an codon-diversified version of Venus/EYFP. Ares (accession number DQ525024) was exchanged for ECFP, which is an codon-diversified version of ECFP. | Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Fluorophore-derived linker shortened. F16A mutation in mglB | Addgene | 2026-10-17 02:36:59 | 0 |
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pRSET FLIPglu-600uDelta11 Aphrodite Resource Report Resource Website 1+ mentions |
RRID:Addgene_13569 | FLIPglu F16A Delta11 Aphrodite | E. coli | Ampicillin | PMID:16935985 | For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html EYFP was exchanged with Aphrodite (accession number DQ525025), which is an codon-diversified version of Venus/EYFP. | Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Fluorophore-derived linker shortened. F16A mutation in mglB | Addgene | 2026-10-17 02:36:59 | 2 |
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pSCC2 Resource Report Resource Website |
RRID:Addgene_39993 | EcoRI endonuclease & EcoRI methylase | E. coli | Ampicillin | PMID:6088551 | pKC30 plasmid derivation is described in Nature (1981) 292, 128-132. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pKC30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | none | Addgene | 2026-10-17 02:45:58 | 0 |
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pLMB51 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40083 | gusA | E. coli | Tetracycline | PMID:22820336 | GenBank accession number JQ895026 Multiple-cloning site (MCS) of pLMB51: GATTACCTCA GTCTAGAGCT CGAGAAGCTT GGATCCATGG TACCCTACA | Vector Backbone:RP4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | Addgene | 2026-10-17 02:45:59 | 1 | |
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pAT253 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40943 | GFP-LacI** | E. coli | Ampicillin | PMID:21724830 | The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 (Addgene plasmid #40942) was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. The LacI** variant was then subcloned into pAFS135 using NotI and XhoI restriction sites to create plasmid pAT253. | Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:4600; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization | Addgene | 2026-10-17 02:46:06 | 1 |
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pJY019 Resource Report Resource Website |
RRID:Addgene_32999 | etpBAC locus | E. coli | Ampicillin | PMID:16552055 | Please note that the His and Myc tags are not in frame with the ORF. In addition, there is a E65G mutation in EtpB. Depositing lab does not think this mutation will affect plasmid function. | Backbone Marker:Invitrogen; Backbone Size:4094; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression, n/a; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:44:58 | 0 | |
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pVS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_33143 | LacO (256 copies) | E. coli | Ampicillin | PMID:18614049 | Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP. | Vector Backbone:Ylplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:45:00 | 2 | |
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EcMBP165-cpGFP.PPYF.T203V.pRSET Resource Report Resource Website |
RRID:Addgene_33373 | Improved MBP based Maltose Biosensor | E. coli | Ampicillin | PMID:21989929 | The GFP-T203V mutation decreases the fluorescence emission of the apo state and increases that of the saturated state in GCaMP2. In the context of MBP165-cpGFP.PPYF, the T203V mutation decreases the fluorescence emission of the apo state by half, whereas saturated fluorescence and affinity are unchanged, increasing ΔF/F to 6.5. | Backbone Marker:Invitrogen; Backbone Size:2300; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | cpGFP146 variant from GCaMP2 inserted between AA165-166 of MBP with T203V mutation in GFP. | Addgene | 2026-10-17 02:45:03 | 0 |
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pAJ1 Resource Report Resource Website |
RRID:Addgene_138379 | RpoA | E. coli | Ampicillin | PMID:31484766 | A novel method for the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking the alpha C-terminal domain.2 Twist KA, Husnain SI, Franke JD, Jain D, Campbell EA, Nickels BE, Thomas MS, Darst SA, Westblade LF Protein Sci. 2011 Jun;20(6):986-95. doi: 10.1002/pro.622. Epub 2011 Apr 26. | Backbone Size:15815; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:37:24 | 0 | |
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pHCKan-mipAp-GFPuv Resource Report Resource Website |
RRID:Addgene_139071 | mipAp-GFPuv | E. coli | Kanamycin | PMID:32353228 | DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid. | Vector Backbone:pSMART-HC Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:37:31 | 0 | |
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pHCKan-phoHp1-GFPuv Resource Report Resource Website |
RRID:Addgene_139075 | phoHp-GFPuv | E. coli | Kanamycin | PMID:32353228 | DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid. | Vector Backbone:pSMART-HC Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:37:31 | 0 | |
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pHCKan-phoUp-GFPuv Resource Report Resource Website |
RRID:Addgene_139076 | phoUp-GFPuv | E. coli | Kanamycin | PMID:32353228 | DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid. | Vector Backbone:pSMART-HC Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:37:31 | 0 | |
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pHCKan-pstSp-GFPuv Resource Report Resource Website |
RRID:Addgene_139077 | pstSp-GFPuv | E. coli | Kanamycin | PMID:32353228 | DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid. | Vector Backbone:pSMART-HC Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:37:31 | 0 | |
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pHCKan-tktBp-GFPuv Resource Report Resource Website |
RRID:Addgene_139081 | tktBp-GFPuv | E. coli | Kanamycin | PMID:32353228 | DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid. | Vector Backbone:pSMART-HC Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:37:31 | 0 |
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