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Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:5087; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21209917
Proper citation: RRID:Addgene_37278 Copy
Genetic Insert: multiple cloning site
Vector Backbone Description: Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20320 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Pimyc-tetR#10
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20318 Copy
Species: Synthetic
Genetic Insert: crRNA to ligD
Vector Backbone Description: Vector Backbone:pMN016; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: Shuttle vector Mycobacterium smegmatis/E.coli
Proper citation: RRID:Addgene_104623 Copy
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104907 Copy
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104912 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cyclin D3;1
Vector Backbone Description: Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31679162
Proper citation: RRID:Addgene_140750 Copy
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:26934495
Proper citation: RRID:Addgene_157876 Copy
Genetic Insert: n/a
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pSMT3; Vector Types:Shuttle vector for mycobacteria; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Comments: Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb) 87: 481–488.
There are known discrepancies between the Addgene QC sequence and the depositor's full sequence--these are likely due to how the full sequence was assembled and should not affect function.
Proper citation: RRID:Addgene_26589 Copy
Species: synthetic gene
Genetic Insert: P#38-luxAB-ssrAec
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27062 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp-ssrAec
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27059 Copy
Species: synthetic gene
Genetic Insert: P#38-gfp-ssrAec
Vector Backbone Description: Backbone Size:5574; Vector Backbone:pTE-0X1-GCT1; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27053 Copy
Species: synthetic gene
Genetic Insert: P#38-luxAB-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27063 Copy
Species: synthetic gene
Genetic Insert: P#38-gfp
Vector Backbone Description: Backbone Size:6055; Vector Backbone:pTE-0X1-GCT5; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP under the control of a strong promoter
Proper citation: RRID:Addgene_27051 Copy
Genetic Insert: tetR(sB), clpXtb
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_27373 Copy
Genetic Insert: scbR
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology, Integrative into Streptomyces genome via serine integrase (like parent pSET152); Bacterial Resistance:Hygromycin
Defining Citation: PMID:29130169
Comments: Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. ‘Orthogonal Regulatory Circuits for Escherichia Coli Based on the γ-Butyrolactone System of Streptomyces Coelicolor’. ACS Synthetic Biology 7, no. 4 (2018): 1043–55. https://doi.org/10.1021/acssynbio.7b00425.
pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase.
Proper citation: RRID:Addgene_172356 Copy
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5304; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
This is a control plasmid for pSC301 in which the SOD promoter was excised.
Proper citation: RRID:Addgene_31852 Copy
Genetic Insert: int L5; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This expression vector integrates at the mycobacteriophage L5 site and can be stabilized by excision of the vector backbone including the int gene using the Cre recombinase; contains xylE gene as a reporter for integration; terminators to protect expression cassette against transcriptional interference.
Proper citation: RRID:Addgene_32376 Copy
Genetic Insert: gfpm2+
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Mycobacterial GFP expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18439872
Comments: This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).
Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554
Proper citation: RRID:Addgene_32362 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Wasabi. The Wasabi ORF is present at bp# 39-749.
Proper citation: RRID:Addgene_30174 Copy
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