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Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Andrew Fire; Vector Backbone:pPD158.87; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2020.11.25.398784 for bioRxiv preprint.
Proper citation: RRID:Addgene_169692 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescue fragment in pDESTR4R3
Vector Backbone Description: Backbone Size:4115; Vector Backbone:pDESTR4R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17247 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9014; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note that there are several mismatches in sequence between depositor's sequence and Addgene's quality control sequence for this plasmid. These mismatches are in backbone regions and should not affect function.
Proper citation: RRID:Addgene_17297 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9773; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17292 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9594; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17293 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9796; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note that Addgene's quality control sequence shows several gaps with depositor's reference sequence. These gaps are in vector region only and should not affect function.
Proper citation: RRID:Addgene_17392 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8832; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17386 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9150; Vector Backbone:pQCXIN; Vector Types:Retroviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17399 Copy
Species:
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:13170; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Parent lentivector, co-expression of Venus selection gene
Proper citation: RRID:Addgene_25734 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:12944; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Parent lentivector, co-expression of Zeocin selection gene
Proper citation: RRID:Addgene_25736 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9377; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.
Proper citation: RRID:Addgene_25890 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25946 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25943 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25945 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25941 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25959 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25960 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25962 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7401; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Use for puromycin selection of transgenes in C. elegans.
This vector is identical to pBCN21-R4R3, but a 8xC183_minPmyo-2::mCherry::unc-54_3'UTR cassette was inserted in the backbone as an additional visual marker.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26302 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8090; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Use for puromycin selection of transgenes in Caenorhabditis species other than C. elegan (works also in C. elegans).
This vector is the same as pBCN22-R4R3 but with a different Pmyo-2::mCherry::unc-54_3'UTR construct that gives good fluoresence expression in the pharynx of C. elegans as well as other species, e.g. C. briggsae, C. remanei and C. brenneri.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26303 Copy
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