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Species: Escherichia coli
Genetic Insert: trpC-tonB
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167591 Copy
Species: Escherichia coli
Genetic Insert: tonB fs
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The frame-shift of the TonB amphipathic helix was produced by deletion of bp 607 (G) and insertion of a new bp (T) following position 636 of the subsequent tonB open reading frame. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167593 Copy
Species: Escherichia coli
Genetic Insert: fepA V15C
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The parent is pKP515 in this collection (PMID: 12218040)
Proper citation: RRID:Addgene_167639 Copy
Species: Escherichia coli
Genetic Insert: fepA I14C
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The parent is pKP515 in this collection (PMID: 12218040)
Proper citation: RRID:Addgene_167638 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167603 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167602 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167601 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167605 Copy
Species: Escherichia coli
Genetic Insert: cba-his6, A121C, Y254C
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The parent plasmid is pKP864 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
Proper citation: RRID:Addgene_167660 Copy
Species: Escherichia coli
Genetic Insert: cba-his6 D17C, M261C
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The parent plasmid is pKP864 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
Proper citation: RRID:Addgene_167653 Copy
Species: Escherichia coli
Genetic Insert: cba-his6 M261C
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The parent plasmid is pKP864 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
Proper citation: RRID:Addgene_167652 Copy
Species: Escherichia coli
Genetic Insert: his6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167651 Copy
Species: Escherichia coli
Genetic Insert: cba-his6
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The His6 tag is located at the C-terminus of colicin B. The parent plasmid is pKP579 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
Proper citation: RRID:Addgene_167658 Copy
Species: Escherichia coli
Genetic Insert: cba-his6 V21C, M261C
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: parent plasmid is pKP579
Proper citation: RRID:Addgene_167657 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167673 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167672 Copy
Species: Escherichia coli
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167671 Copy
Species: Escherichia coli
Genetic Insert: E. coli SRP RNA
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Also referenced in: Watters, K., Strobel, E., Yu, A. et al. Cotranscriptional folding of a riboswitch at nucleotide resolution. Nat Struct Mol Biol 23, 1124–1131 (2016). https://doi.org/10.1038/nsmb.3316.
Please visit https://www.biorxiv.org/content/10.1101/379222v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162240 Copy
Species: Escherichia coli
Genetic Insert: GlpG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_155133 Copy
Species: Escherichia coli
Genetic Insert: GlpG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_155134 Copy
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