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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
iLID-eGFP-C2
 
Resource Report
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RRID:Addgene_114415 iLID domain Synthetic Kanamycin PMID:30182785 Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:00:09 0
pAAVS1-TRE-MA4-GFP
 
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RRID:Addgene_114380 MLL-AF4 fusion gene Homo sapiens Ampicillin PMID:29386396 Backbone Marker:Rudolf Jaenisch, Addgene Plasmid #22075; Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:09 0
Anti-Synaptotagmin-12 [N277/7R]
 
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RRID:Addgene_114482 anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750763. Isotype: IgG2a. Additional species: mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
Anti-SUR2B [N323B/20R]
 
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RRID:Addgene_114480 anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750775. Isotype: IgG2a. Additional species: rat, mouse, human. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
Anti-Botch [N116/14R]
 
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RRID:Addgene_114487 anti-Botch (Rattus norvegicus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750705. Isotype: IgG2a. Additional species: rat, mouse, human. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
Anti-Flrt3 [N353/13R]
 
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RRID:Addgene_114486 anti-Flrt3 (Mus musculus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750784. Isotype: IgG2a. Additional species: mouse. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
AICSDP-29: LMNB1-mTagRFP-T
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_114403 LMNB1 Homology Arms with mTagRFPT-linker Homo sapiens Ampicillin This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human LMNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQAS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used 2026-08-01 01:00:09 1
pAB(EXPR-HDAC5-trun1)
 
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RRID:Addgene_114400 PYL-HDAC5 aa 1-275 Homo sapiens Ampicillin PMID:34494094 Backbone Size:4977; Vector Backbone:pAAV-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:09 0
AICSDP-58: NUP153-mEGFP
 
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RRID:Addgene_114407 NUP153 Homology Arms with mEGFP-linker Homo sapiens Ampicillin This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human NUP153 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: YSDLE. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used 2026-08-01 01:00:09 0
AICSDP-57: SMC1A-mEGFP
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_114406 SMC1A Homology Arms with linker-mEGFP Homo sapiens Ampicillin This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SMC1A in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: LSSSGPSGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used 2026-08-01 01:00:09 2
Anti-Kirrel3, short and long [N321C/49R]
 
Resource Report
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RRID:Addgene_114490 anti-Kirrel3, short and long (Rattus norvegicus) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750774. Isotype: IgG2a. Additional species: rat, mouse. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
pAAV-hSyn-mCherry
 
Resource Report
Resource Website
100+ mentions
RRID:Addgene_114472 mCherry Other Ampicillin Backbone Marker:Stratagene; Backbone Size:4537; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 103
pAAV-Ef1a-fDIO mCherry
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_114471 mCherry Other Ampicillin Backbone Marker:Stratagene; Backbone Size:5603; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 29
Anti-Ankyrin-R [N388A/10R]
 
Resource Report
Resource Website
RRID:Addgene_114479 anti-Ankyrin-R (Homo sapiens) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750789. Isotype: IgG2a. Additional species: human, mouse, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
Anti-GABA(A)R, Epsilon [N413/67R]
 
Resource Report
Resource Website
RRID:Addgene_114478 anti-GABA(A)R, Epsilon (Homo sapiens) recombinant mouse monoclonal antibody Mus musculus Ampicillin PMID:30667360 RRID: AB_2750793. Isotype: IgG2a. Additional species: human, rat. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
pIAGO-Hap2
 
Resource Report
Resource Website
RRID:Addgene_114460 Hap2 + perDII Other Ampicillin PMID:20189107 This plasmid encodes an AmphDI-PerDI hybrid glycosyltransferase and a PerDII perosamine synthase. Vector Backbone:pIAGO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
PLL7.0 tgRFP cPYP
 
Resource Report
Resource Website
RRID:Addgene_114464 cPYP Other Ampicillin PMID:30203962 Plasmid lacks features necessary for lentiviral production, but should be unaffected for mammalian expression of tgRFP cPYP. Vector Backbone:PLL 7.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin M121E 2026-08-01 01:00:10 0
pAAV-CaMKIIa-mCherry
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_114469 mCherry Other Ampicillin Backbone Marker:Stratagene; Backbone Size:5372; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 42
pTriEX Tom20 mVenus BoPL
 
Resource Report
Resource Website
RRID:Addgene_114467 BoPL Synthetic Ampicillin PMID:30203962 Backbone Size:5886; Vector Backbone:pTriEX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0
pVL-FLAG/His LCOR
 
Resource Report
Resource Website
RRID:Addgene_114450 LCOR Homo sapiens Ampicillin PMID:29628311 Vector Backbone:pVL FLAG/His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:10 0

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