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Species: Homo sapiens
Genetic Insert: CD44
Vector Backbone Description: Vector Backbone:pHL-Sec; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Gileadi lab uses HEK293 cells for expression.
Proper citation: RRID:Addgene_175453 Copy
Species: Homo sapiens
Genetic Insert: CNN3
Vector Backbone Description: Vector Backbone:pSUMO-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning using SUMO-LIC5 and SUMO-LIC3 sites. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175456 Copy
Species: Homo sapiens
Genetic Insert: H2B-HaloTag10 and Tomm20-HaloTag9
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Comments: Please note there are an additional 35 amino acids at the end of the insert. Depositor confirms these do not affect plasmid function. This plasmid was used in the experiments discussed in their publication.
Proper citation: RRID:Addgene_175525 Copy
Species: Homo sapiens
Genetic Insert: Lifeact-HaloTag11 and Tomm20-HaloTag7
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175526 Copy
Species: Homo sapiens
Genetic Insert: NES-HaloTag7
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175527 Copy
Species: Homo sapiens
Genetic Insert: Cox8a-HaloTag7
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175529 Copy
Species: Homo sapiens
Genetic Insert: SYK
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Gileadi lab uses BL21(DE3)-R3-pRARE3 cells for expression.
Proper citation: RRID:Addgene_175488 Copy
Species: Homo sapiens
Genetic Insert: H2B-HaloTag10
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175521 Copy
Species: Homo sapiens
Genetic Insert: H2B-HaloTag11
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175522 Copy
Species: CoV2
Genetic Insert: K164A/H165A nsp1 CoV2
Vector Backbone Description: Backbone Marker:GE (cytiva); Backbone Size:4950; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175514 Copy
Species: CoV2
Genetic Insert: C_K nsp1 CoV2
Vector Backbone Description: Backbone Marker:GE (cytiva); Backbone Size:4950; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175517 Copy
Species: Adenovirus
Genetic Insert: Ad VAI RNA
Vector Backbone Description: Backbone Size:3117; Vector Backbone:pUC; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175518 Copy
Species: Homo sapiens
Genetic Insert: HaloTag11
Vector Backbone Description: Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175519 Copy
Species: CoV2
Genetic Insert: nsp1 CoV2
Vector Backbone Description: Backbone Marker:GE (cytiva); Backbone Size:4950; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175512 Copy
Species: Homo sapiens
Genetic Insert: Beta4Gal-HaloTag9
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175546 Copy
Species: Homo sapiens
Genetic Insert: H2B-HaloTag7 and Tomm20-HaloTag9
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175547 Copy
Species: Mus musculus
Genetic Insert: WAPL
Vector Backbone Description: Vector Backbone:pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33318687
Proper citation: RRID:Addgene_175549 Copy
Species: Homo sapiens
Genetic Insert: LAMP1-HaloTag9
Vector Backbone Description: Vector Backbone:pCDNA5/FR/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_175541 Copy
Species: Mus musculus
Genetic Insert: Agrp
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16604086
Proper citation: RRID:Addgene_17552 Copy
Species: Mus musculus
Genetic Insert: Pomc promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16604086
Comments: Addgene's sequence shows a C->T mutation in nt 129 of POMC. The depositing scientist is aware of this mutation and has found that it does not alter the promoter activity.
Proper citation: RRID:Addgene_17553 Copy
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