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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF
 
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RRID:Addgene_148686 HsGIGYF2_35-105-E46AE47FN91A Homo sapiens Ampicillin PMID:28698298 sequence of NCBI: NM_015575.3 or Uniprot.: Q6Y7W6-1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-08 01:09:36 0
pnEA-NpM-Scp20_18-49-GB1_AF
 
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RRID:Addgene_148720 Scp20_18-49 Saccharomyces cerevisiae Ampicillin PMID:30053226 Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-08 01:09:36 0
pT7-V5-SBP-C1-HsGIGYF1_1-671_AI
 
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RRID:Addgene_148962 HsGIGYF1_1-671 Homo sapiens Kanamycin PMID:31439631 sequence extracted from NCBI: NM_022574.4 or Uniprot.: O75420 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-08 01:09:36 0
pEGFP-N3-FLuc-K0-Rluc_AH
 
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RRID:Addgene_148846 K0-Rluc Other Kanamycin reporter for Ribosome Quality control Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-08 01:09:36 0
pJRL2 GAL1pr VYFP (EB#1571)
 
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RRID:Addgene_14847 GAL1 promoter Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-08 01:09:35 0
pYL192 (TRV1)
 
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1+ mentions
RRID:Addgene_148968 TRV RNA1 Tobacco Rattle Virus RNA 1 Kanamycin PMID:12028572 Backbone Size:11498; Vector Backbone:pBIN19; Vector Types:Plant vector with TRV RNA1; this vector used with pYL156(TRV RNA2) for virus induced gene silencing (VIGS); Bacterial Resistance:Kanamycin P697S mutation in putative replicase. S239G and S246P mutation in 16kDa protein. 2026-08-08 01:09:36 9
pYL156 (TRV RNA2)
 
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1+ mentions
RRID:Addgene_148969 Modified TRV RNA2 Modified Tobacco Rattle Virus RNA 2 Kanamycin PMID:12028572 There are a few differences between the Addgene full sequence and the Genbank ID: AF406991. These discrepancies were confirmed to not cause functional differences: - 1 bp insertion (T3313) in backbone. - 1 bp mismatch (T5593C) and 1 bp insertion (G5646) in pVS1 RepA (known variant). - IS4-like element inserted between ori and KanR (1338bp insert at positions 8295-9632bp). Backbone Size:6426; Vector Backbone:pCAMBIA0390 T-DNA vector; Vector Types:T-DNA vector; Bacterial Resistance:Kanamycin see comments section below 2026-08-08 01:09:36 5
pLCAG EGFP
 
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1+ mentions
RRID:Addgene_14857 EGFP Ampicillin PMID:11854510 CAG promoter was cloned into the ClaI and BamHI sites of LV-pGFP, replacing the PGK promoter. Backbone Marker:Naldini; Backbone Size:0; Vector Backbone:LV-pGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-08 01:09:36 4
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)
 
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RRID:Addgene_14852 GAL1 promoter TATA-C2 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-C2. TATA box mutated from TATATAAG to TCTATAAG. 2026-08-08 01:09:35 0
Lox-stop-Lox p53 R270H
 
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RRID:Addgene_14853 p53 R270H Mus musculus Ampicillin PMID:15607980 Conditional mutant alleles of p53 were generated by introducing the LoxP-flanked transcriptional STOP cassette into intron 1 of the p53 gene. Site-directed mutagenesis was used to introduce an arg→his mutation at codon 270. Note: This is a large plasmid that can undergo recombination. Bacterial colonies will grow slowly. If there are different colony sizes on a plate, the smaller ones are more likely to contain the correct plasmid. Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin Contact mutant R270H. (Murine p53 codon 270 corresponds to human codon 273). 2026-08-08 01:09:35 0
pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)
 
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RRID:Addgene_14851 GAL1 promoter TATA-C2 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-C2. TATA box mutated from TATATAAG to TCTATAAG. 2026-08-08 01:09:35 0
pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AE
 
Resource Report
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RRID:Addgene_148575 HsGIGYF1_1-177-YYMLtoAAAA Homo sapiens Kanamycin PMID:28698298 sequence extracted from NCBI: NM_022574.4 or Uniprot.: O75420 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-08 01:09:36 0
pGBW-m4046459
 
Resource Report
Resource Website
RRID:Addgene_148971 SARS-CoV-2 nsp10 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-08 01:09:36 0
pGBW-m4046567
 
Resource Report
Resource Website
RRID:Addgene_148972 SARS-CoV-2 RNA-dependent RNA polymerase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-08 01:09:36 0
pGBW-m4046435
 
Resource Report
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RRID:Addgene_148974 SARS-CoV-2 helicase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725308.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-08 01:09:36 0
pGBW-m4046380
 
Resource Report
Resource Website
RRID:Addgene_148977 SARS-CoV-2 E (envelope) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-08 01:09:36 0
pGBW-m4046160
 
Resource Report
Resource Website
RRID:Addgene_148978 SARS-CoV-2 nsp4 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-08 01:09:36 0
pHR' CMV GFP
 
Resource Report
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1+ mentions
RRID:Addgene_14858 CMV-GFP Ampicillin PMID:9294208 HIV vector with CMV promoter. Constructed by inserting the CMV-GFP fragment from pKS-CMV-GFP into the same sites of pHR'. Backbone Size:0; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-08 01:09:36 6
pnEA-NpH-HsNot9_19-285-V181E_AE
 
Resource Report
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RRID:Addgene_148617 HsNot9_19-285-V181E Homo sapiens Ampicillin PMID:28165457 sequence based on NCBI:NM_005444.1. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-08 01:09:36 0
pnEA-NpM-DmeIF4G_601-660-GB1_AD
 
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RRID:Addgene_148506 DmeIF4G_601-660 Drosophila melanogaster Ampicillin PMID:27773676 ORF extracted from NCBI: NM_079901.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-08 01:09:35 0

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