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Species: Homo sapiens
Genetic Insert: HsGIGYF2_35-105-E46AE47FN91A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28698298
Comments: sequence of NCBI: NM_015575.3 or Uniprot.: Q6Y7W6-1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148686 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Scp20_18-49
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30053226
Comments: Geneart clone of ScCaf20p, codon optimized for Expression in E.coli. (sequence according to Uniprot.: P12962) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148720 Copy
Species: Homo sapiens
Genetic Insert: HsGIGYF1_1-671
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31439631
Comments: sequence extracted from NCBI: NM_022574.4 or Uniprot.: O75420 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148962 Copy
Species: Other
Genetic Insert: K0-Rluc
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: reporter for Ribosome Quality control Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148846 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
The GAL1 promoter was amplified from genomic DNA.
Proper citation: RRID:Addgene_14847 Copy
Species: Tobacco Rattle Virus RNA 1
Genetic Insert: TRV RNA1
Vector Backbone Description: Backbone Size:11498; Vector Backbone:pBIN19; Vector Types:Plant vector with TRV RNA1; this vector used with pYL156(TRV RNA2) for virus induced gene silencing (VIGS); Bacterial Resistance:Kanamycin
Defining Citation: PMID:12028572
Proper citation: RRID:Addgene_148968 Copy
Species: Modified Tobacco Rattle Virus RNA 2
Genetic Insert: Modified TRV RNA2
Vector Backbone Description: Backbone Size:6426; Vector Backbone:pCAMBIA0390 T-DNA vector; Vector Types:T-DNA vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12028572
Comments: There are a few differences between the Addgene full sequence and the Genbank ID: AF406991. These discrepancies were confirmed to not cause functional differences:
- 1 bp insertion (T3313) in backbone.
- 1 bp mismatch (T5593C) and 1 bp insertion (G5646) in pVS1 RepA (known variant).
- IS4-like element inserted between ori and KanR (1338bp insert at positions 8295-9632bp).
Proper citation: RRID:Addgene_148969 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Naldini; Backbone Size:0; Vector Backbone:LV-pGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11854510
Comments: CAG promoter was cloned into the ClaI and BamHI sites of LV-pGFP, replacing the PGK promoter.
Proper citation: RRID:Addgene_14857 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter TATA-C2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
The GAL1 promoter was amplified from genomic DNA.
Proper citation: RRID:Addgene_14852 Copy
Species: Mus musculus
Genetic Insert: p53 R270H
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15607980
Comments: Conditional mutant alleles of p53 were generated by introducing the LoxP-flanked transcriptional STOP cassette into intron 1 of the p53 gene. Site-directed mutagenesis was used to introduce an arg→his mutation at codon 270.
Note: This is a large plasmid that can undergo recombination. Bacterial colonies will grow slowly. If there are different colony sizes on a plate, the smaller ones are more likely to contain the correct plasmid.
Proper citation: RRID:Addgene_14853 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter TATA-C2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
The GAL1 promoter was amplified from genomic DNA.
Proper citation: RRID:Addgene_14851 Copy
Species: Homo sapiens
Genetic Insert: HsGIGYF1_1-177-YYMLtoAAAA
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28698298
Comments: sequence extracted from NCBI: NM_022574.4 or Uniprot.: O75420 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148575 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148971 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 RNA-dependent RNA polymerase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148972 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 helicase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725308.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 E (envelope)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148977 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp4
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_148978 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9294208
Comments: HIV vector with CMV promoter. Constructed by inserting the CMV-GFP fragment from pKS-CMV-GFP into the same sites of pHR'.
Proper citation: RRID:Addgene_14858 Copy
Species: Homo sapiens
Genetic Insert: HsNot9_19-285-V181E
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28165457
Comments: sequence based on NCBI:NM_005444.1. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148617 Copy
Species: Drosophila melanogaster
Genetic Insert: DmeIF4G_601-660
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27773676
Comments: ORF extracted from NCBI: NM_079901.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148506 Copy
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