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Species: Rattus norvegicus
Genetic Insert: Syntaxin2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:PCDNA4/TO/myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12737809
Proper citation: RRID:Addgene_14817 Copy
Species: Homo sapiens
Genetic Insert: MKK3
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4952; Vector Backbone:pGex 3x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7839144
Proper citation: RRID:Addgene_14814 Copy
Species: Rattus norvegicus
Genetic Insert: DAG reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: CFP and YFP fused to the C1A and C1B domains of PKCbetaII. DAGR was created by PCR of the C1A and C1B domains of rat PKCßII (aa 37–152) from KpnI to XhoI and substitution for the PH domain in CYPHR.
Proper citation: RRID:Addgene_14865 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: Phosphoacceptor threonine in the PKC substrate sequence changed to alanine. PKC substrate sequence flanked by flexible linker sequence: GGSGG RFRRFQALKIKAKA GGSGG. See CKAR for additional information.
Proper citation: RRID:Addgene_14861 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: The MyrPalm tag in this plasmid uses the Lyn kinase amino terminal sequence of MGCIKSK from UniProtKB reference sequence P07948.3. This sequence encodes for myristoylation and palmitoylation, shown previously to be sufficient to target a protein to the plasma membrane.
Proper citation: RRID:Addgene_14862 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: PKC substrate sequence flanked by flexible linker sequence: GGSGG RFRRFQTLKIKAKA GGSGG. A206K mutation present in both CFP (mCFP) and citrine (mYFP) to reduce the intrinsic homoaffinity of all GFPs and preclude intermolecular FRET by CFP-YFP dimerization. CFP was amplified by PCR from a plasmid template to encode a HindIII restriction site followed by a consensus initiation site for translation (CGCCACC) before the initiating ATG of CFP, and a KpnI restriction site at the 3' end instead of a terminating codon. FHA2, a gift from Michael Yaffe (MIT, Cambridge, MA) was amplified by PCR to include KpnI and BamHI restriction sites at the 5' and 3' ends, respectively. Citrine was amplified by PCR to include a 5' BamHI followed by the PKC substrate sequence and a 3' XbaI following the terminating codon.
Proper citation: RRID:Addgene_14860 Copy
Species: Homo sapiens
Genetic Insert: HsRck_296-472-10xmut
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27009120
Comments: ORF extracted from NCBI: NM_004397.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148420 Copy
Species: Homo sapiens
Genetic Insert: HseIF4E
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27773676
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148669 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: NucCKAR was generated by fusing sequences encoding a basic nuclear localization signal, PKKRKVEDA, to the 3'-end of CKAR .
Proper citation: RRID:Addgene_14869 Copy
Genetic Insert: B kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15583002
Comments: PKB substrate sequence: RKRDRLGTLGI.
Proper citation: RRID:Addgene_14877 Copy
Species: Rattus norvegicus
Genetic Insert: DAG binding domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: YFP-tagged DAG binding domain (YFP-DBD) was first constructed by fusion of monomeric YFP to the 5'-end of the C1b domain of PKCbeta. This construct was improved for DAG binding by introducing the Y123W point mutation via QuikChange (Stratagene).
Proper citation: RRID:Addgene_14874 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: MitoCKAR was generated by fusing in frame sequences encoding the amino-terminal 33 amino acids of TOM 20 to the immediate 5'-end of CKAR.
Proper citation: RRID:Addgene_14872 Copy
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: Sequences encoding the amino-terminal 35 residues of endothelial nitric-oxide synthase were fused in frame to the 5'-end of CFP.
Proper citation: RRID:Addgene_14873 Copy
Genetic Insert: C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16901905
Comments: CytoCKAR was generated by fusing a region encoding the nuclear export sequence, LALKLAGLDI, to the 3'-end of CKAR immediately preceding the termination codon.
Proper citation: RRID:Addgene_14870 Copy
Species: Other
Genetic Insert: 6xbsMS2-random
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27009120
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148431 Copy
Species: Homo sapiens
Genetic Insert: HsHelz
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31570513
Comments: sequence according to the sequence of NCBI:NM_014877.3 or Uniprot.:P42694 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148799 Copy
Species: Drosophila melanogaster
Genetic Insert: DmBam_141-422
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29255063
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148833 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO5 promoter UASm1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15166317
Comments: pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript.
The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector.
Proper citation: RRID:Addgene_14841 Copy
Species: Homo sapiens
Genetic Insert: Hs4E-T-shRNA
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27009120
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148445 Copy
Species: Homo sapiens
Genetic Insert: Hs4EHP_52-234opt
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28698298
Comments: Geneart clone of Hs4EHP codon optimized for expression in E.coli. sequence. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148564 Copy
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