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Species: Homo sapiens
Genetic Insert: E2F3
Vector Backbone Description: Vector Backbone:pWPI-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907129
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
Proper citation: RRID:Addgene_114298 Copy
Species: Synthetic
Genetic Insert: Promoter J23116
Vector Backbone Description: Backbone Size:2210; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649439, Alternative ID = pGT327 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114331 Copy
Species: Homo sapiens
Genetic Insert: E2F2
Vector Backbone Description: Vector Backbone:pWPI-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907129
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
Proper citation: RRID:Addgene_114297 Copy
Species: T. maritima
Genetic Insert: fatty acid binding protein
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12577257
Comments: Please note that the plasmid pSKB3.TM1468 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11431 Copy
Species: Synthetic
Genetic Insert: Promoter J23113
Vector Backbone Description: Backbone Size:2210; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649438, Alternative ID = pGT328 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114330 Copy
Species: Synthetic
Genetic Insert: RBS RBSc58
Vector Backbone Description: Backbone Size:2210; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649446, Alternative ID = pGT333 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114336 Copy
Species: Synthetic
Genetic Insert: RBS RBSc33
Vector Backbone Description: Backbone Size:2210; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649442, Alternative ID = pGT331 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114334 Copy
Species: Synthetic
Genetic Insert: Terminator L3S2P55
Vector Backbone Description: Backbone Size:2206; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649450, Alternative ID = pGT337 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114339 Copy
Species: Mycoplasma pneumoniae M129
Genetic Insert: methenyltetrahydrofolate synthetase
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15281135
Comments: Please note that the plasmid pSKB3.MPN348 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11437 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114285 Copy
Species: Homo sapiens
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114284 Copy
Species: Homo sapiens
Genetic Insert: hM3Dq
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29079819
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114283 Copy
Vector Backbone Description: Vector Backbone:pCWX; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
Proper citation: RRID:Addgene_114321 Copy
Vector Backbone Description: Vector Backbone:pCWX; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
Proper citation: RRID:Addgene_114320 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin Receptor
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114286 Copy
Species: Synthetic
Genetic Insert: Promoter J23107
Vector Backbone Description: Backbone Size:2210; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649437, Alternative ID = pGT326 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114329 Copy
Species: Synthetic
Genetic Insert: Promoter J23100
Vector Backbone Description: Backbone Size:2210; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649435, Alternative ID = pGT323 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114327 Copy
Species: Mus musculus
Genetic Insert: BDNF
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Subcloned from Addgene Plasmid #39857. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114292 Copy
Species: Homo sapiens
Genetic Insert: rERK2 and hMEK1 proteins
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: rat ERK2 fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES. Subcloned from Addgene plasmid #39197. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114291 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114290 Copy
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