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Genetic Insert: GtACR2-EYFP-TlcnC
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30091701
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/25/331165.1 for bioRxiv preprint.
Proper citation: RRID:Addgene_114374 Copy
Species: Other
Genetic Insert: rtTA and BGH-pA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5255; Vector Backbone:pTet-On Advanced; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20974949
Comments: This construct is also called CAG-rtTA and has been used in our recent published paper ID 29386396.
Proper citation: RRID:Addgene_114379 Copy
Genetic Insert: GtACR2-KA2N-EGFP
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30091701
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/25/331165.1 for bioRxiv preprint.
Proper citation: RRID:Addgene_114378 Copy
Species: Synthetic
Genetic Insert: 12X601-MA3
Vector Backbone Description: Vector Backbone:pENTR223; Vector Types:Mammalian Expression, Unspecified; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30455303
Proper citation: RRID:Addgene_114360 Copy
Species: Synthetic
Genetic Insert: 3xNLS_SpCas9
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-21 a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30911135
Comments: This plasmid is also associated with the following publication:
PMID 30911135: Highly efficient therapeutic gene editing of human hematopoietic stem cells
Proper citation: RRID:Addgene_114365 Copy
Species: Synthetic
Genetic Insert: 36X601-MA1_3_2
Vector Backbone Description: Vector Backbone:pENTR223; Vector Types:Mammalian Expression, Unspecified; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30455303
Proper citation: RRID:Addgene_114364 Copy
Species: Synthetic
Genetic Insert: 36X601-MA1_2_3
Vector Backbone Description: Vector Backbone:pENTR223; Vector Types:Mammalian Expression, Unspecified; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30455303
Proper citation: RRID:Addgene_114363 Copy
Genetic Insert: ReaChR-P2A-dTomato
Vector Backbone Description: Backbone Size:5324; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30091701
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/25/331165.1 for bioRxiv preprint.
Proper citation: RRID:Addgene_114368 Copy
Genetic Insert: hChR2(H134R)
Vector Backbone Description: Backbone Size:4815; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30091701
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/25/331165.1 for bioRxiv preprint.
Proper citation: RRID:Addgene_114367 Copy
Species: Synthetic
Genetic Insert: 12X601-MA1
Vector Backbone Description: Vector Backbone:pENTR223; Vector Types:Mammalian Expression, Unspecified; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30455303
Proper citation: RRID:Addgene_114358 Copy
Species: Synthetic
Genetic Insert: Terminator L3S2P21
Vector Backbone Description: Backbone Size:2206; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649449, Alternative ID = pGT338 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114340 Copy
Species: Other
Genetic Insert: crtI (codon optimised)
Vector Backbone Description: Backbone Size:2209; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Alternative ID = pGT356 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114343 Copy
Species: Synthetic
Genetic Insert: Terminator ECK120033737
Vector Backbone Description: Backbone Size:2206; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649448, Alternative ID = pGT339 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114341 Copy
Species: Homo sapiens
Genetic Insert: E2F1
Vector Backbone Description: Vector Backbone:pWPI-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907129
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
Proper citation: RRID:Addgene_114296 Copy
Species: Mus musculus
Genetic Insert: Itch
Vector Backbone Description: Backbone Marker:see PMID 1852137; Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11724934
Proper citation: RRID:Addgene_11434 Copy
Species: Other
Genetic Insert: dGFP
Vector Backbone Description: Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25732825
Comments: Subcloned from Addgene Plasmid #17114
Proper citation: RRID:Addgene_114295 Copy
Species: M. pneumoniae
Genetic Insert: cell division protein
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15146477
Comments: Please note that the plasmid pSKB3.MPN314 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11433 Copy
Species: E. coli
Genetic Insert: OsmC
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15103136
Comments: Please note that the plasmid pSKB3.EG10680 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11436 Copy
Species: Synthetic
Genetic Insert: RBS RBSc13
Vector Backbone Description: Backbone Size:2210; Vector Backbone:pGT400; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30462270
Comments: Accession Number MG649441, Alternative ID = pGT330 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114333 Copy
Species: Homo sapiens
Genetic Insert: TFDP1
Vector Backbone Description: Vector Backbone:pWPI-DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23907129
Comments: Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
Proper citation: RRID:Addgene_114299 Copy
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