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Genetic Insert: multiple cloning site
Vector Backbone Description: Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20320 Copy
Species: Mus musculus
Genetic Insert: cMYC
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:8376; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371436
Proper citation: RRID:Addgene_20324 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:8376; Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371436
Proper citation: RRID:Addgene_20322 Copy
Species: Synthetic
Genetic Insert: Perceval (fluorescent ATP:ADP sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2764; Vector Backbone:pRset B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19122669
Proper citation: RRID:Addgene_20336 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20298 Copy
Species: Mus musculus
Genetic Insert: Klf4-F2A-Oct4
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19109433
Proper citation: RRID:Addgene_20331 Copy
Species: A. victoria
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20296 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:9250; Vector Backbone:pLenti-CaMKIIa; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079251
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20295 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20299 Copy
Species: Mus musculus
Genetic Insert: Klf4-F2A-cMyc
Vector Backbone Description: Backbone Marker:homemade; Backbone Size:7222; Vector Backbone:FUW (Lenti-Lox-Ubi); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19109433
Proper citation: RRID:Addgene_20332 Copy
Species: Homo sapiens
Genetic Insert: lamin A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15750600
Comments: To generate the wt lamin A minigene, intron 11 was first introduced between exon 11 and exon 12 in the lamin A cDNA in pEGFP-lamin A using a multi-step PCR reaction. In the fist step a region of the lamin A gene containing the last 156 bp of exon 11, intron 11 and the first 31 bp of exon 12 was amplified by PCR from genomic DNA obtained from HeLa cells, using the primers: LMNA-mini11wtF (5’-GCCCAGGTGGGCGGACCCATC-3’)/LMNA-mini12R (5’-CAGATTACATGATGCTGCAGTTCTG-3’). In the second step, a region of pEGFP-lamin A from the last 37 bp of exon 9 to the mutated region in exon 11 was amplified using the primers LMNA-mini9F/LMNA-mini11wtR (5’-GATGGGTCCGCCCACCTGGGC-3’). In the third step a region of pEGFP-lamin A containing the last 240 bp of exon 12 was amplified using the primers: LMNA-mini12F (5’-CAGAACTGCAGCATCATGTAATCTG-3’)/ GFPvectorR. The PCR products obtained from the first and third steps were mixed and used in a fourth PCR reaction in the presence of the primers LMNA-mini11wtF/GFPvectorR. The obtained PCR product was then mixed with the product obtained from the second step and used in a fifth PCR reaction with the primers: LMNA-mini9F/ GFPvectorR. The final amplification product was cloned into the BsiWI/XbaI site of pEGFP-lamin A to obtain wt pEGFP-lamin Aintr11. To generate the minigene wt pEGFP-lamin Aintr11 was digested with EcoRI and BstWI enzymes to remove a region of lamin A cDNA from exon 1 to the first 91 bp of exon 9, filled with Klenow enzyme and self-ligated. The mutated minigene was generated with the same procedure, substituting primers LMNA-mini11wtF e LMNA-mini11wtR respectively with: LMNA-mini11mutF (5’-GCCCAGGTGGGTGGACCCATC-3’) and LMNA-mini11mut R (5’-GATGGGTCCACCCACCTGGGC-3’).
Proper citation: RRID:Addgene_20292 Copy
Genetic Insert: UAS cassette (GAL1,10)
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: Cassette: UAS;
UAS: GAL1,10;
X: UbiY;
Linker: N/A
Proper citation: RRID:Addgene_20385 Copy
Genetic Insert: UAS cassette (FUS1)
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: Cassette: UAS;
UAS: FUS1;
X: UbiY;
Linker: N/A
Proper citation: RRID:Addgene_20384 Copy
Genetic Insert: UAS cassette (FUS1)
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: Cassette: UAS;
UAS: FUS1;
X: UbiM;
Linker: N/A
Proper citation: RRID:Addgene_20383 Copy
Species: Cloning vector
Genetic Insert: ECFPpA-ACN
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2394; Vector Backbone:pSP72; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18546598
Proper citation: RRID:Addgene_20301 Copy
Genetic Insert: CFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: CFP-reporter;
UAS: GAL1,10;
X: UbiY;
Linker: none
Proper citation: RRID:Addgene_20389 Copy
Genetic Insert: CFP-reporter
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16598699
Comments: See attachments for details
Cassette: CFP-reporter;
UAS: GAL1,10;
X: UbiM;
Linker: none
Proper citation: RRID:Addgene_20388 Copy
Genetic Insert: mir21(P) targets
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pCMV-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12554881
Proper citation: RRID:Addgene_20382 Copy
Species: E. coli
Genetic Insert: Pmyc1tetO-lacZ
Vector Backbone Description: Backbone Size:4103; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_20315 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Pimyc-tetR#10
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20318 Copy
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