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Vector Backbone Description: Backbone Size:8174; Vector Backbone:pLKO.3G; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.
pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.
Proper citation: RRID:Addgene_14748 Copy
Species: Homo sapiens
Genetic Insert: HsNot2_344-540
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121232
Comments: ORF extracted from NCBI: NM_014515.5. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147618 Copy
Genetic Insert: membrane bound form of EGFP
Vector Backbone Description: Backbone Size:6122; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus.
The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments.
Addgene's NGS finds an IS4-like element (ISVsa5 family transposase) in the backbone that does not affect the overall plasmid function.
Proper citation: RRID:Addgene_14757 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta K85A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8382613
Comments: Nru I site is added to the K->A mutation
Proper citation: RRID:Addgene_14755 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flpe-ERT2 fusion protein
Vector Backbone Description: Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation.
Proper citation: RRID:Addgene_14756 Copy
Species: Homo sapiens
Genetic Insert: HsNot2-F502A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24121232
Comments: ORF extracted from NCBI: NM_014515.5. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147501 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta S9A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7980435
Comments: S2A in the sequence does not affect function, used to introduce restriction enzyme site
Proper citation: RRID:Addgene_14754 Copy
Species: N. pharaonis
Genetic Insert: Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9500; Vector Backbone:FCK(1.3)GW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17375185
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_14750 Copy
Species: Homo sapiens
Genetic Insert: N-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Proper citation: RRID:Addgene_14724 Copy
Species: Homo sapiens
Genetic Insert: N-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Proper citation: RRID:Addgene_14725 Copy
Species: Homo sapiens
Genetic Insert: K-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Proper citation: RRID:Addgene_14722 Copy
Species: Homo sapiens
Genetic Insert: H-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Comments: GTPase deficient (dominant active) mutant: GAP insensitive, increased GDP/GTP exchange, and chronically GTP bound; transforming.
Proper citation: RRID:Addgene_14720 Copy
Species: Homo sapiens
Genetic Insert: H-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Comments: Prenylation mutant: modified by geranylgeranylation, still undergoies remaining CAAX-signaled modifications and palmitoylation; retains transforming activity.
Proper citation: RRID:Addgene_14721 Copy
Species: Caenorhabditis elegans
Genetic Insert: CeAIN2-W366AW390A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22402495
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147235 Copy
Species: Drosophila melanogaster
Genetic Insert: DmRpl10
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: ORF extracted from variant C NCBI: NM_143697.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147350 Copy
Vector Backbone Description: Backbone Size:4644; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD93.51, Ligation number NONE.
Proper citation: RRID:Addgene_1474 Copy
Vector Backbone Description: Backbone Size:6794; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD93.97, Ligation number NONE.
Proper citation: RRID:Addgene_1476 Copy
Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD94.81, Ligation number NONE.
Proper citation: RRID:Addgene_1477 Copy
Vector Backbone Description: Backbone Size:4444; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD91.14, Ligation number NONE.
Proper citation: RRID:Addgene_1472 Copy
Vector Backbone Description: Backbone Size:4644; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1995. Fire Lab Miniprep Number pPD93.48, Ligation number NONE.
Proper citation: RRID:Addgene_1473 Copy
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