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Species: Mus musculus
Genetic Insert: Magoh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pCMV-Sport6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: AluI digestion generates 341 bp, 189 bp, 112 bp. Wt only generates 341 bp +300 bp fragments.
Proper citation: RRID:Addgene_30481 Copy
Species: Mus musculus
Genetic Insert: Bmi1
Vector Backbone Description: Backbone Size:4918; Vector Backbone:pCS2+ att-cherry-HA (C-term cherry-HA); Vector Types:Mammalian Expression, Xenopus/Zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30516 Copy
Species: Mus musculus
Genetic Insert: macroH2A1.2-C
Vector Backbone Description: Vector Backbone:pDEST pCS2+ att-eGFP-HA (C term eGFP-HA); Vector Types:Mammalian Expression, Xenopus/Zebrafish expression.; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30515 Copy
Species: Mus musculus
Genetic Insert: mH2A2 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6343; Vector Backbone:pSUPER retro puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: mH2A2 shRNA: 5’-GCAGAGGACAAGAAGCTTAAA
Proper citation: RRID:Addgene_30518 Copy
Species: Mus musculus
Genetic Insert: mH2A1 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6343; Vector Backbone:pSUPER retro puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: H2A1 shRNA: 5’-GGGTGCAGACAAATGTGAA
Proper citation: RRID:Addgene_30517 Copy
Species: Mus musculus
Genetic Insert: Sin3A
Vector Backbone Description: Backbone Size:4354; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30452 Copy
Species: Mus musculus
Genetic Insert: Sin3A
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pME18S; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_30454 Copy
Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The construct was created by recovering the Sma1 fragment of mMyD116 and ligating it into the unique SnB1 site of pBABEpu. The stop codo is deep in the downstream SV40 promoter sequence andowing the encoded poly-peptide with a tail of nonesense sequence, but it does serve as an expressed negative control for the effects of GADD34.
Proper citation: RRID:Addgene_21835 Copy
Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:4724; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21834 Copy
Species: Mus musculus
Genetic Insert: XBP-1 processed/spliced
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V.
FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon.
Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.
Proper citation: RRID:Addgene_21833 Copy
Species: Mus musculus
Genetic Insert: XBP-1 unprocessed
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G27V and A76V.
FLAG-epitope tagged unspliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon.
Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.
Proper citation: RRID:Addgene_21832 Copy
Species: Mus musculus
Genetic Insert: PSMB2
Vector Backbone Description: Backbone Size:5921; Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21798 Copy
Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21796 Copy
Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21795 Copy
Species: Mus musculus
Genetic Insert: PERK (537-1114)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse PERK (537-1114), wild-type, 9E10-tagged at C-terminus, fused to GST, bacterial expression vector, pGEX4T-1
Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.
Proper citation: RRID:Addgene_21817 Copy
Species: Mus musculus
Genetic Insert: PERK (C-term deleted)
Vector Backbone Description: Backbone Size:4756; Vector Backbone:pCDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse PERK, C-term deleted (removes amino-acids 582-1081), 9E10-tagged at C-terminus, mammalian expression vector.
Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.
Proper citation: RRID:Addgene_21816 Copy
Species: Mus musculus
Genetic Insert: PSMD14
Vector Backbone Description: Backbone Marker:Clontech (?); Backbone Size:4200; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that though there is a Kan resistance gene in this plasmid according to the author's sequence, the gene is not functional. This plasmid does NOT confer Kan resistance.
Proper citation: RRID:Addgene_21801 Copy
Species: Mus musculus
Genetic Insert: PSMD14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21800 Copy
Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse IRE1b, wild-type, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3.
Proper citation: RRID:Addgene_21880 Copy
Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)
Proper citation: RRID:Addgene_21857 Copy
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