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Genetic Insert: I-SceI nuclease
Vector Backbone Description: Backbone Marker:Daniel Aubert; Backbone Size:7360; Vector Backbone:pDA17 (Broad host range plasmid recovered form Bordetella bronchiseptica); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18341581
Proper citation: RRID:Addgene_32061 Copy
Species: Homo sapiens
Genetic Insert: DUSP7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_27978 Copy
Species: Homo sapiens
Genetic Insert: DUSP7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_27976 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Comments: Y244C mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510
Proper citation: RRID:Addgene_27985 Copy
Vector Backbone Description: Backbone Size:8136; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11483365
Comments: A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3929. The depositing scientist says this deletion has no impact on the function of the plasmid.
Proper citation: RRID:Addgene_28037 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_208896 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_203678 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_203675 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671
Proper citation: RRID:Addgene_203673 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205019 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205017 Copy
Species: E. coli
Genetic Insert: T7 polymerase
Vector Backbone Description: Backbone Size:8751; Vector Backbone:pSW213; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36645284
Comments: The T7 polymerase gene (1) was cloned from the chromosome of BL21(DE3).
Proper citation: RRID:Addgene_192982 Copy
Vector Backbone Description: Backbone Marker:Spaink; Backbone Size:10400; Vector Backbone:pMP103; Vector Types:Bacterial Expression, IncP; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24276795
Comments: the polylinker of pIC20H is broadly available and isn't covered by an MTA
Proper citation: RRID:Addgene_185921 Copy
Species: Synthetic
Genetic Insert: lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
Vector Backbone Description: Vector Backbone:pFW11 Tet; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37406096
Proper citation: RRID:Addgene_199119 Copy
Species: Synthetic
Genetic Insert: LanYFP
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_201856 Copy
Genetic Insert: Tetracycline Resistance Gene
Vector Backbone Description: Backbone Size:790; Vector Backbone:R6Kγ origin of replication and oriT; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37712562
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543168v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_203960 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Comments: The user should always streak the culture on plate and work with isolated fluorescent colonies. Addgene NGS results found mixed population of nucleotides across Term_apFAB390 and C-terminus of NeonGreen, but plasmid function should not be affected.
Proper citation: RRID:Addgene_201855 Copy
Vector Backbone Description: Vector Backbone:pFE604T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38724726
Proper citation: RRID:Addgene_202601 Copy
Vector Backbone Description: Backbone Marker:Host-Microbe Interactions lab; Backbone Size:2437; Vector Backbone:pUdO1te; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38235654
Proper citation: RRID:Addgene_210242 Copy
Vector Backbone Description: Backbone Marker:Host-Microbe Interactions lab; Backbone Size:3241; Vector Backbone:pUdO4te; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38235654
Proper citation: RRID:Addgene_210245 Copy
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