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Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29388 Copy
Genetic Insert: Amber-Cerulean-Venus-Amber
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3940; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Stoichiometry standard; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19946626
Comments: The 1st Amber can be excised using a NheI-BSP E1 double digest; Cerulean by BSP E1-BglII; Venus by BglII-SalII and the last (2nd) Amber by SalI-BamHI. The full insert can be released by a NheI-BamHI double-digest.
Due to the multiple fluorescent protein repeats in this plasmid, Addgene is unable to confirm the entire insert by sequencing. Please see Addgene's diagnostic digest for this plasmid by clicking the Notes from Addgene link above.
Proper citation: RRID:Addgene_29421 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29387 Copy
Genetic Insert: Amber-Cerulean-Amber-Venus
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3940; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Stoichiometry standard; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19946626
Comments: The 1st Amber can be excised using a NheI-BSP E1 double digest; Cerulean by BSP E1-BglII; the 2nd Amber by BglII-SalI and Venus by SalI-BamHI. The full insert can be released by a NheI-BamHI double-digest.
Due to the multiple fluorescent protein repeats in this plasmid, Addgene is unable to confirm the entire insert by sequencing. Please see Addgene's diagnostic digest for this plasmid by clicking the Notes from Addgene link above.
Proper citation: RRID:Addgene_29420 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29384 Copy
Species: Rattus norvegicus
Genetic Insert: TSC2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14651849
Proper citation: RRID:Addgene_29360 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15790532
Proper citation: RRID:Addgene_29357 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15790532
Proper citation: RRID:Addgene_29354 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-5X-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15790532
Proper citation: RRID:Addgene_29353 Copy
Species: Homo sapiens
Genetic Insert: CCNA2 LONG 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2 (Promega); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19748357
Proper citation: RRID:Addgene_29472 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA-DEST40; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29407 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA-DEST40; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29406 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA-DEST40; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29404 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA-DEST40; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29403 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7464; Vector Backbone:pEF-DEST51; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_29401 Copy
Species: Mus musculus
Genetic Insert: SEMA3A
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:for ISH; Bacterial Resistance:Ampicillin
Comments: Please note that this vector was designed to generate a probe for in situ hybridization (ISH) and is functional for this purpose. The SEMA3A sequence has not been verified to be full-length and may contain additional mutations when compared to the GenBank reference sequence.
Proper citation: RRID:Addgene_29450 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-5X-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15790532
Proper citation: RRID:Addgene_29350 Copy
Species: Homo sapiens
Genetic Insert: E2F2 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2 (Promega); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19748357
Proper citation: RRID:Addgene_29469 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGW1myc2b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12851414
Proper citation: RRID:Addgene_29347 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA3.1/GS; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:12851414
Proper citation: RRID:Addgene_29345 Copy
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