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Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101815 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101816 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101811 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101821 Copy
Species: Synthetic
Genetic Insert: 3xERSE-I
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.29; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32577017
Proper citation: RRID:Addgene_101789 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101804 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:8690; Vector Backbone:pMuLE Dest PuroR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101893 Copy
Species: Synthetic
Genetic Insert: PA-mCherry
Vector Backbone Description: Backbone Marker:SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.; Backbone Size:7500; Vector Backbone:pSin4-EF2-IRES-Pur; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30983487
Comments: There is a "NheI" restriction site which we artificially added between PAmCherry and SNCA-NE to facilitate cloning. i.e. 5'---PAmCherry-NheI-SNCA-NE----3'
This plasmid encodes a novel 18-amino-acid protein tag - "NE", which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)
Proper citation: RRID:Addgene_102364 Copy
Species: Synthetic
Genetic Insert: PA-mCherry
Vector Backbone Description: Backbone Marker:SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.; Backbone Size:7500; Vector Backbone:pSin4-EF2-IRES-Pur; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30983487
Comments: "KFERQ" is the consensus peptide sequence found in various native proteins for mediating degradation via Chaperone-Mediated Autophagy (CMA)
There is a "NheI" restriction site which we artificially added between PAmCherry and KFERQ-NE to facilitate cloning. i.e. 5'---PAmCherry-NheI-KFERQ-NE----3'
This plasmid encodes a novel 18-amino-acid protein tag - "NE", which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)
Proper citation: RRID:Addgene_102365 Copy
Species: Synthetic
Genetic Insert: TurboRFP
Vector Backbone Description: Backbone Marker:Goodrum Lab, University of Arizona; Backbone Size:7353; Vector Backbone:pCIG3 #78264; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30263951
Proper citation: RRID:Addgene_102343 Copy
Species: Synthetic
Genetic Insert: 7SK paired gRNA insert
Vector Backbone Description: Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30346403
Proper citation: RRID:Addgene_102440 Copy
Species: Synthetic
Genetic Insert: LbCpf1
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29222508
Comments: Please visit https://doi.org/10.1101/156125 for bioRxiv preprint.
Proper citation: RRID:Addgene_102566 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102667 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102669 Copy
Species: Synthetic
Genetic Insert: HaloTag-bActinCDS-bActin3'UTR-MS2V5
Vector Backbone Description: Backbone Size:6250; Vector Backbone:pUbC; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27791158
Comments: Please note, due to the AT rich sequence within the Actin 3'UTR, there are some discrepancies between the Addgene NGS result and the provided full plasmid reference sequence.
Proper citation: RRID:Addgene_102718 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102670 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102672 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102674 Copy
Species: Synthetic
Genetic Insert: Cys-SpyCatcher002
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:6422; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29024296
Proper citation: RRID:Addgene_102829 Copy
Species: Synthetic
Genetic Insert: Sr22 PI190945
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32779520
Proper citation: RRID:Addgene_102836 Copy
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