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Species:
Genetic Insert: sgRNA for APs5
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66097 Copy
Species: Other
Genetic Insert: CYP119
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).
Proper citation: RRID:Addgene_66131 Copy
Species:
Genetic Insert: sgRNA for APs5
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66095 Copy
Species:
Genetic Insert: sgRNA for APs6
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66094 Copy
Species:
Genetic Insert: sgRNA for APs6
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66092 Copy
Species: Synthetic
Genetic Insert: DNA polymerase sliding clamp 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5698; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The PCNA1 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_66139 Copy
Species:
Genetic Insert: sgRNA for CSD 53
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66099 Copy
Species: Synthetic
Genetic Insert: DNA polymerase sliding clamp 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5698; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The PCNA1 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_66140 Copy
Species: Other
Genetic Insert: STp
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5996; Vector Backbone:pBR325; Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol
References:
Comments: Contains the E. coli STp gene probe (157 bp) cloned on PstI linkers into pBR325 Heat stable enterotoxin gene (porcine)
Proper citation: RRID:Addgene_66149 Copy
Species: Mus musculus
Genetic Insert: SIRT2
Vector Backbone Description: Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66144 Copy
Species: Other
Genetic Insert: eae
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
References:
Comments: E. coli E2348/69 cloned intimin. HindIII restriction site added to the ends of eae gene (1 kb) by PCR and cloned into pUC19.
Proper citation: RRID:Addgene_66150 Copy
Species: Other
Genetic Insert: tdh1
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Tetracycline
References:
Comments: Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1 cloned into the PstI site of pBR322.
Proper citation: RRID:Addgene_66158 Copy
Species: Other
Genetic Insert: tdh2
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
References:
Comments: A 2.7 kb HindIII fragment carrying the 2nd tdh gene copy was cloned from the chromosome of Vibrio parahemolyticus strain WP1 into the HindIII site of pBR322
Proper citation: RRID:Addgene_66159 Copy
Species: Other
Genetic Insert: LEE probe A rorf1, espG
Vector Backbone Description: Backbone Size:4109; Vector Backbone:pSPORT1; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
References:
Comments: E. coli E2348, 3.1 Kb Bg1II-MluI fragment from the left end of the LEE region cloned into the BamHI-MluI site of vector pSPORT I. Contains rorf1 and espG. parent strain: E. coli E2348/69
Proper citation: RRID:Addgene_66154 Copy
Species: Other
Genetic Insert: espA
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3461; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: 6XHis N-terminal tagged espA pQE30 (Qiagen). Must be grown in strain that carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome (eg XL1-Blue).
Proper citation: RRID:Addgene_66155 Copy
Species: Mus musculus
Genetic Insert: FoxO1 KA
Vector Backbone Description: Vector Backbone:pBabe bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66164 Copy
Species: Other
Genetic Insert: ctxB
Vector Backbone Description: Backbone Marker:Kaper lab, Addgene plasmid 63737; Vector Backbone:pCVD315; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
References:
Comments: ctxB with KpnI linkers cloned into pCVD315 KpnI site. 1.4 kb ctxB probe.plasmid GENOTYPE :pCVD315::ctxB
Proper citation: RRID:Addgene_66162 Copy
Species: Mus musculus
Genetic Insert: SIRT3
Vector Backbone Description: Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66167 Copy
Species: Mus musculus
Genetic Insert: FoxO1 KR
Vector Backbone Description: Vector Backbone:pBabe bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66166 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVA; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: parts.igem.org/Part:pSB1A2
Proper citation: RRID:Addgene_66053 Copy
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