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Species: Synthetic
Genetic Insert: pCMV(MinDis)-iCyt_BrEt_SnFR_PM
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177747 Copy
Species: Homo sapiens
Genetic Insert: BSD-based EXSISERS
Vector Backbone Description: Vector Backbone:pBlueScript-KS II (+); Vector Types:Mammalian Expression, CRISPR, Luciferase, Synthetic Biology, Unspecified; Bacterial Resistance:Ampicillin
References:
Comments: HMGU has filed a patent (WO2020161236A1 “Detection of splice events of a gene of interest”) disclosing the capabilities of EXSISERS, and retains ownership rights to EXSISERS Material.
Proper citation: RRID:Addgene_177867 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-psm (gagctcACTATACAACCTACAAGCACAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17773 Copy
Species: Synthetic
Genetic Insert: pCMV(MinDis)-iCytSnFR_ER
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177742 Copy
Species: Homo sapiens
Genetic Insert: SMARCC1
Vector Backbone Description: Backbone Size:4775; Vector Backbone:pFAST BAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177863 Copy
Species: Drosophila melanogaster
Genetic Insert: cg11094 utr fragment
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17777 Copy
Species: Synthetic
Genetic Insert: iCytSnFR
Vector Backbone Description: Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177738 Copy
Species: Mus musculus
Genetic Insert: Egr2-AS-RNA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8745; Vector Backbone:pLVX-DsRed-Express2-N1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177737 Copy
Species: Synthetic
Genetic Insert: iCyt_F_SnFR
Vector Backbone Description: Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177739 Copy
Species: Drosophila melanogaster
Genetic Insert: cg1849 utr fragment mut
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17780 Copy
Species: Drosophila melanogaster
Genetic Insert: cg5166a/c utr fragment
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17781 Copy
Species: Homo sapiens
Genetic Insert: AMPK (a1b2g1)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_177850 Copy
Species: Drosophila melanogaster
Genetic Insert: cg6551 utr fragment
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17783 Copy
Species: Other
Genetic Insert: FlucDM
Vector Backbone Description: Vector Backbone:p406 TDH3; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177731 Copy
Species: Drosophila melanogaster
Genetic Insert: cg6551 utr fragment mut
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17784 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid was designed to tag transcripts of insert at the 3' with an 12X MS2 stem loop tag. Insertion of the transcript of interst is performed by BsaI cloning.
5' BsaI generates ACAG overhang (BsaI recognition site 2659-2664)
3' BsaI generates CCCG overhang (BsaI recognition site 4086-4091)
These BsaI sites are lost upon insertion of the transcript of interest.
Proper citation: RRID:Addgene_177809 Copy
Species: Homo sapiens
Genetic Insert: GRHL1
Vector Backbone Description: Vector Backbone:pcDNA3.1/Hygro(+) (Thermo Fisher Scientific, #V87020); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177769 Copy
Species: Synthetic
Genetic Insert: KRaION2
Vector Backbone Description: Backbone Size:4005; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
Proper citation: RRID:Addgene_177802 Copy
Species: Homo sapiens
Genetic Insert: GRHL1
Vector Backbone Description: Vector Backbone:pcDNA3.1/Hygro(+) (Thermo Fisher Scientific, #V87020); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177768 Copy
Species: Mus musculus
Genetic Insert: miR-9/9*
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177805 Copy
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