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Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: This construct was produced through de novo gene synthesis.
Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66101 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology, Other, Destination Vector; Bacterial Resistance:Kanamycin
References:
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66069 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chronos
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: This construct was produced through de novo gene synthesis.
Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66102 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Synthetic Biology, Other, Destination Vector; Bacterial Resistance:Kanamycin
References:
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66067 Copy
Species:
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:2455; Vector Backbone:pAH63; Vector Types:; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_66073 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: parts.igem.org/Part:pSB1K3
Proper citation: RRID:Addgene_66071 Copy
Species: Synthetic
Genetic Insert: None
Vector Backbone Description: Vector Backbone:DVK; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_66072 Copy
Species: Other
Genetic Insert: Nef
Vector Backbone Description: Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66079 Copy
Species:
Genetic Insert: phi80 integrase
Vector Backbone Description: Vector Backbone:pAH123; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66077 Copy
Species: Synthetic
Genetic Insert: Second generation Rac1 FRET biosensor
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4900; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66110 Copy
Species: Homo sapiens
Genetic Insert: NMT
Vector Backbone Description: Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66078 Copy
Species:
Genetic Insert: sgRNA for APs4
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66090 Copy
Species:
Genetic Insert: sgRNA for APa4-2
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66086 Copy
Species:
Genetic Insert: sgRNA for APs1
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66087 Copy
Species: Drosophila melanogaster
Genetic Insert: Ncd kinesin-14 motor protein
Vector Backbone Description: Backbone Size:7814; Vector Backbone:pCaSpeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Other references describing Ncd-GFP expression:
Endow SA & Komma DJ 1996 J Cell Sci 109, 2429-2442 - PubMed ID: 8923204
Skold HN, Komma DJ & Endow SA 2005 J Cell Sci 118, 1745-1755 - PubMed ID: 15797926
Proper citation: RRID:Addgene_66082 Copy
Species: Mus musculus
Genetic Insert: G protein alpha-q-EE-YFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66080 Copy
Species: Mus musculus
Genetic Insert: G protein alpha-q-EE-CFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_66081 Copy
Species: Sulfolobus solfataricus P2
Genetic Insert: DNA polymerase sliding clamp 1
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2206; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
References:
Comments: We cloned the PCNA1 gene from the cell suspension of S. solfataricus P2 strain (ATCC35092).
Proper citation: RRID:Addgene_66125 Copy
Species:
Genetic Insert: sgRNA for APs1
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66088 Copy
Species:
Genetic Insert: sgRNA for APs4
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_66089 Copy
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