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Species: Drosophila melanogaster
Genetic Insert: dme-miR-1006 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments: Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro.
Proper citation: RRID:Addgene_17760 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-miR-1003 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments: Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro.
Proper citation: RRID:Addgene_17761 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-let-7 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments: let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin and cloned into pMT-puro.
Proper citation: RRID:Addgene_17764 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7 perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
References:
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-ps (gagctcACTATACAACCTACTACCTCAactagt) Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17766 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-let-7 minigene
Vector Backbone Description: Backbone Marker:Carl Thummel Lab; Backbone Size:10200; Vector Backbone:pCaSpeR4-tubulin promoter; Vector Types:Drosophila P-element vector; Bacterial Resistance:Ampicillin
References:
Comments: let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites.
Proper citation: RRID:Addgene_17769 Copy
Species: Homo sapiens
Genetic Insert: GRHL1
Vector Backbone Description: Vector Backbone:pcDNA3.1/Hygro(+) (Thermo Fisher Scientific, #V87020); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177772 Copy
Species: Homo sapiens
Genetic Insert: GRHL
Vector Backbone Description: Vector Backbone:pGL4.27 (Promega Corporation, #E8451); Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177774 Copy
Species: Homo sapiens
Genetic Insert: GRHL1
Vector Backbone Description: Vector Backbone:pcDNA3.1/Hygro(+) (Thermo Fisher Scientific, #V87020); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177773 Copy
Species: Homo sapiens
Genetic Insert: NRF2
Vector Backbone Description: Vector Backbone:pGL4.27 (Promega Corporation, #E8451); Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177775 Copy
Species: Homo sapiens
Genetic Insert: CopGFP inserted behind human synapsin I promoter
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177810 Copy
Species: Arabidopsis thaliana
Genetic Insert: SNF1-related protein kinase 2.3
Vector Backbone Description: Backbone Marker:Life Sensors; Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177846 Copy
Species: Arabidopsis thaliana
Genetic Insert: SNF1-related protein kinase 2
Vector Backbone Description: Backbone Marker:Life Sensors; Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177845 Copy
Species: Other
Genetic Insert: hFluc
Vector Backbone Description: Backbone Marker:Addgene #177714; Vector Backbone:phFluc-WT; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177727 Copy
Species: Arabidopsis thaliana
Genetic Insert: PYR1-like 2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_177842 Copy
Species: Homo sapiens
Genetic Insert: Folate receptor alpha
Vector Backbone Description: Vector Backbone:pCDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: IgK = Ig kappa leader, th = thrombin cleavage site, Fc = Igl Fc fragment
Proper citation: RRID:Addgene_177841 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10151; Vector Backbone:pDEST-DB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177796 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10394; Vector Backbone:pDEST-AD-CYH2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177795 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10164; Vector Backbone:pDEST-DB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177798 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10388; Vector Backbone:pDEST-AD-CYH2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177797 Copy
Species: Synthetic
Genetic Insert: KRaION1
Vector Backbone Description: Backbone Size:4002; Vector Backbone:pBAD-HisD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
Proper citation: RRID:Addgene_177799 Copy
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