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Genetic Insert: RLuc
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11509180
Proper citation: RRID:Addgene_124893 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31069270
Proper citation: RRID:Addgene_124890 Copy
Genetic Insert: Cas12a
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31069270
Proper citation: RRID:Addgene_124891 Copy
Vector Backbone Description: Vector Backbone:pZHY988; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30928637
Comments: Plant codon optimized,R1335V/L1111R/D1135V/G1218R/E1219F/A1322R/T1337R
Proper citation: RRID:Addgene_124895 Copy
Species: Rattus norvegicus
Genetic Insert: rat IgG2a Hinge-G4S-Sortag-Histag
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3956; Vector Backbone:pCR4 TOPO TA; Vector Types:Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124810 Copy
Species: Homo sapiens
Genetic Insert: AR LBD
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:pSP72; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12888538
Proper citation: RRID:Addgene_12492 Copy
Species: Bacteria
Genetic Insert: iNicSnFR3b-(V7)
Vector Backbone Description: Backbone Marker:Janelia; Backbone Size:2827; Vector Backbone:pHHM.X513; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30718376
Comments: Vector was received from Janelia HHMI
Proper citation: RRID:Addgene_124881 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Addgene#50469; Vector Backbone:pAAV-CaMKII-EGFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32025031
Comments: bioRxiv pre-print 10.1101/579250
Proper citation: RRID:Addgene_124882 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pMCSV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969085
Comments: Guide RNA to be cloned into BbsI site. Supplied modified golden-gate cloning method is required given that extra BbsI site is located within the 2A sequence.
To design guide RNAs:
-add ACCG to 5' end of positive strand (positive strand defined as strand which will contact PAM sequence at 3' end)
-add AAAC to 5' end of negative strand
Proper citation: RRID:Addgene_124889 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7162; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124887 Copy
Species: Homo sapiens
Genetic Insert: MLST8
Vector Backbone Description: Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124915 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagGFP2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124919 Copy
Species: Homo sapiens
Genetic Insert: MLST8
Vector Backbone Description: Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124918 Copy
Species: Mus musculus
Genetic Insert: Gabrg2
Vector Backbone Description: Vector Backbone:pX601; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209486
Proper citation: RRID:Addgene_124871 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124911 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124912 Copy
Species: Homo sapiens
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3519; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31548418
Proper citation: RRID:Addgene_124876 Copy
Species: Mus musculus
Genetic Insert: Mlst8
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124910 Copy
Species: Homo sapiens
Genetic Insert: MLST8
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124905 Copy
Species: Mus musculus
Genetic Insert: p65 promoter- p65 CDS-mRuby2
Vector Backbone Description: Backbone Size:7206; Vector Backbone:pLenti _Blast_DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30904375
Proper citation: RRID:Addgene_124903 Copy
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