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Species: Mus musculus
Genetic Insert: anti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877197. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177455 Copy
Species: Hyphochytrium catenoides
Genetic Insert: HcKCR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177336 Copy
Species: SARS-CoV-2 virus
Genetic Insert: SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Silent mutation in the original sequence to delete a second NdeI site as NdeI was needed for cloning; codon coding for Q306 was mutated to codon coding for A306 to delete natural cleavage site of the 3CL-protease; codon coding for active site residue C145 was mutated to A145 for inactive protease production; Factor Xa cleavage site can cleave off all 3 downstream tags; three tandem FLAG epitope tags is followed by an enterokinase cleavage site.
Proper citation: RRID:Addgene_177335 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in pharyngeal muscle. Inject at 2.5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW77 has 11 random mutations relative to the pCFJ90 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177338 Copy
Species: Mus musculus
Genetic Insert: anti-Parvalbumin (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877608. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177459 Copy
Species: Mus musculus
Genetic Insert: anti-Homer1L (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877572. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177458 Copy
Species: Homo sapiens
Genetic Insert: Cyclin-dependent kinase 1
Vector Backbone Description: Backbone Size:5334; Vector Backbone:pF1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Genes are gene-optimised for insect cell expression and have been ordered from GeneArts.
Proper citation: RRID:Addgene_177330 Copy
Species:
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Synthetic Biology, Cell-Free Protein Synthesis; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_177369 Copy
Species: Mus musculus
Genetic Insert: anti-GAD65/67 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2756510. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177474 Copy
Species: Mus musculus
Genetic Insert: anti-Synapsin-3 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2725817. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177471 Copy
Species: Mus musculus
Genetic Insert: anti-Pan-Synapsin (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2728740. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177472 Copy
Species: Mus musculus
Genetic Insert: anti-NCKX4 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877624. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177549 Copy
Species: Mus musculus
Genetic Insert: anti-Kv3.2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877619. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177548 Copy
Species: Mus musculus
Genetic Insert: anti-Cav1.3 Ca2+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877308. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177540 Copy
Species: Mus musculus
Genetic Insert: anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877419. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177542 Copy
Species: Mus musculus
Genetic Insert: anti-GIT1 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877278. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177545 Copy
Species: Mus musculus
Genetic Insert: anti-GABA(A)R, Alpha2 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877202. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177544 Copy
Species: Mus musculus
Genetic Insert: anti-Npas4 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877229. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177547 Copy
Species: Mus musculus
Genetic Insert: anti-MAP3K12 (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877434. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177538 Copy
Species: Mus musculus
Genetic Insert: anti-SUR2A (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877363. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177530 Copy
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