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Species: Mus musculus
Genetic Insert: anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877311. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177447 Copy
Species: Homo sapiens
Genetic Insert: S6K2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17729 Copy
Species: Mus musculus
Genetic Insert: anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877334. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177438 Copy
Species: Synthetic
Genetic Insert: RFP, CauNEO
Vector Backbone Description: Backbone Size:2683; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177277 Copy
Species: Methanogenic archaeon ISO4-G1
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:2452; Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_177310 Copy
Species: Methanogenic archaeon ISO4-G1
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:2452; Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_177311 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA_RNH1_RNH201
Vector Backbone Description: Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177271 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4179; Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177270 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pRS415U; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177272 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:T7p:cgRNA_HIS3_stop:tZ
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pEDJ400; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177275 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:HIS3_23∆29-XII-5 :<-PGK1p
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCfB2909; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Integrative plasmid
Please note: Plasmid contains a 68bp insertion in mADH1t compared to the depositor's provided sequence. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_177274 Copy
Species: Aquifex aeolicus
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:Waugh lab; Backbone Size:8264; Vector Backbone:pKM596; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid produces an MBP-NusG-His6 fusion protein with a recognition site for TVMV protease (ETVRFQS) between MBP and NusG-His6.
See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.
Proper citation: RRID:Addgene_17728 Copy
Species: Mus musculus
Genetic Insert: anti-NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877607. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177464 Copy
Species: Mus musculus
Genetic Insert: anti-VGAT (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877230. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177463 Copy
Species: Mus musculus
Genetic Insert: anti-nNOS/NOS1 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877235. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177466 Copy
Species: Other
Genetic Insert: Aequorin
Vector Backbone Description: Vector Backbone:pGFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177340 Copy
Species: Mus musculus
Genetic Insert: anti-NPY/Neuropeptide Y (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877570. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177461 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in body wall muscle. Inject at 5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW79 has 10 random mutations relative to the pCFJ104 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177339 Copy
Species: Mus musculus
Genetic Insert: anti-VIP (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877573. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177453 Copy
Species: Mus musculus
Genetic Insert: anti-Neuroligin-2 (Mus musculus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877595. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177452 Copy
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