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Species: Homo sapiens
Genetic Insert: S6K2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: V390M is present, but should not affect protein function
Proper citation: RRID:Addgene_17730 Copy
Species: Mus musculus
Genetic Insert: STIM1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1 myc his; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse Stim1 (BglII/NotI, NotI is treated by Klenow) was subcloned into BamHI/XbaI (XbaI is treated by Klenow) of pcDNA3.1myc-His vector.
Proper citation: RRID:Addgene_17732 Copy
Species: Mus musculus
Genetic Insert: STIM1
Vector Backbone Description: Backbone Size:2200; Vector Backbone:Modified pENTR11; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Mouse Stim1 (BglII/NotI) was subcloned into BglII/EagI followed by a myc tag.
Proper citation: RRID:Addgene_17733 Copy
Species: Mus musculus
Genetic Insert: STIM2
Vector Backbone Description: Backbone Size:2200; Vector Backbone:Modified pENTR11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_17734 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Size:7900; Vector Backbone:pCLNRX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17735 Copy
Species:
Genetic Insert: NLS-Cre recombinase
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS-; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: The Cre expression plasmid pOG231 was prepared by fusing a Cre coding sequence modified from pIC-Cre, and containing the same translation start and nuclear localization signal, to the synthetic intron and cytomegalovirus promoter of pOG44
Proper citation: RRID:Addgene_17736 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177261 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Vector Backbone:LentiCRISPRv2puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177259 Copy
Species:
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:7312; Vector Backbone:PQCXP; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17740 Copy
Species: Homo sapiens
Genetic Insert: PIK3R1-Ni
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17744 Copy
Species:
Genetic Insert: Trigger RNA
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Synthetic Biology, Cell-Free Protein Synthesis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177370 Copy
Species: Mus musculus
Genetic Insert: mouse CD8-GFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17745 Copy
Species: Other
Genetic Insert: CgLUCopt-mut2
Vector Backbone Description: Vector Backbone:pVS20; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177373 Copy
Species: Homo sapiens
Genetic Insert: Cyclin-dependent kinase 1
Vector Backbone Description: Backbone Size:6424; Vector Backbone:pF1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Genes are gene-optimised for insect cell expression and have been ordered from GeneArts.
Proper citation: RRID:Addgene_177329 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Gether lab - modified from Addgene plasmid #55641; Backbone Size:4820; Vector Backbone:Addgene plasmid #154867; Vector Types:Mammalian Expression, AAV, FLP-FRT; Bacterial Resistance:Ampicillin
References:
Comments: Control plasmid for Flp-dependent DREADD vectors
Proper citation: RRID:Addgene_177328 Copy
Species: Mus musculus
Genetic Insert: anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2893259. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177449 Copy
Species: E. coli
Genetic Insert: SoxS
Vector Backbone Description: Vector Backbone:p15A; Vector Types:; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_177323 Copy
Species: Mus musculus
Genetic Insert: anti-CASPR2 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877287. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177446 Copy
Species:
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177324 Copy
Species: Mus musculus
Genetic Insert: anti-SK2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a recombinant antibody derived from RRID: AB_2877597. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177448 Copy
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