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Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65596 Copy
Species: E. coli
Genetic Insert: FadD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65595 Copy
Species: Synthetic
Genetic Insert: attB-mCherry
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, Mouse Targeting, Bxb1; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65529 Copy
Species: Synthetic
Genetic Insert: attB-mCherry
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, Mouse Targeting, Bxb1; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65528 Copy
Species: Hepatitis B virus
Genetic Insert: HBV polymerase protein
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65520 Copy
Species: Staphylococcus aureus
Genetic Insert: mammalian codon-optimized SaCas9, and SaCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65770 Copy
Species: Homo sapiens
Genetic Insert: histone H2B
Vector Backbone Description: Backbone Marker:Addgene #64308; Backbone Size:5600; Vector Backbone:pONL-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65704 Copy
Species: Mus musculus
Genetic Insert: Rab11a
Vector Backbone Description: Backbone Marker:Addgene #64308; Backbone Size:5600; Vector Backbone:pONL-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65707 Copy
Species: Mus musculus
Genetic Insert: Rab11a
Vector Backbone Description: Backbone Marker:Addgene #64307; Backbone Size:5600; Vector Backbone:pCNL-C1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65706 Copy
Species: Homo sapiens
Genetic Insert: cytochrome c oxidase subunit VIII
Vector Backbone Description: Backbone Marker:Addgene #64303; Backbone Size:5600; Vector Backbone:pYNL-N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65669 Copy
Species: Homo sapiens
Genetic Insert: RBPMS
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65664 Copy
Species: Homo sapiens
Genetic Insert: RBPMS
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65663 Copy
Species: Mus musculus
Genetic Insert: murine Oct4 promoter and CNL
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:5800; Vector Backbone:pRedZeo; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65718 Copy
Species: Mus musculus
Genetic Insert: murine Nanog promoter and YNL
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:5800; Vector Backbone:pRedZeo; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65717 Copy
Species: Mus musculus
Genetic Insert: beta-tubulin class V
Vector Backbone Description: Backbone Marker:Addgene #64304; Backbone Size:5600; Vector Backbone:pCNL-N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65679 Copy
Species: Synthetic
Genetic Insert: 7xTcf, minCMV, 3xNLS, CNL
Vector Backbone Description: Backbone Marker:Agilent Biotechnology, National Institute of Genetics (Dr Koichi Kawakami Lab); Backbone Size:7700; Vector Backbone:pBluescript II SK(+) based Tol2 donor vector; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65712 Copy
Species: Mus musculus
Genetic Insert: beta-tubulin class V
Vector Backbone Description: Backbone Marker:Addgene #64303; Backbone Size:5600; Vector Backbone:pYNL-N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65678 Copy
Species: Synthetic
Genetic Insert: 7xTcf, minCMV, 3xNLS, YNL
Vector Backbone Description: Backbone Marker:Agilent Biotechnology, National Institute of Genetics (Dr Koichi Kawakami Lab); Backbone Size:7700; Vector Backbone:pBluescript II SK(+) based Tol2 donor vector; Vector Types:Luciferase, Tol2 system; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65711 Copy
Species: Mus musculus
Genetic Insert: murine Sox2 promoter and ONL
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:5200; Vector Backbone:pGreenFire1; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_65719 Copy
Species: Synthetic
Genetic Insert: peroxisomal targeting signal 2
Vector Backbone Description: Backbone Marker:Addgene #64303; Backbone Size:5600; Vector Backbone:pYNL-N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_65675 Copy
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