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Vector Backbone Description: Backbone Size:4525; Vector Backbone:p1NIL; Vector Types:Suicide vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10931901
Proper citation: RRID:Addgene_20187 Copy
Species: Homo sapiens
Genetic Insert: FKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of FKBP
Proper citation: RRID:Addgene_20220 Copy
Species: Mus musculus
Genetic Insert: Notch 3 Intracellular Domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3
(5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
Proper citation: RRID:Addgene_20185 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of FRB
Proper citation: RRID:Addgene_20225 Copy
Species: Homo sapiens
Genetic Insert: 2xFRB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and two repeats of FRB
Proper citation: RRID:Addgene_20224 Copy
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pBR322; Vector Types:Marker cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10931901
Proper citation: RRID:Addgene_20189 Copy
Species: Homo sapiens
Genetic Insert: 3xFKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and three repeats of FKBP
Proper citation: RRID:Addgene_20222 Copy
Species: Mus musculus
Genetic Insert: Notch 2 intracellular domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD2 was cloned into XbaI/BamHI sites. NICD2 (Met-1699 to Ala-2470 of full-length mouse N2) was amplified from the N2 plasmid (gift from Dr. Y. Hamada) using primers 5XbaN2 (5'-GCTCTAGAATGGCCAAGCGGAAGCGCAAG-3') and 3BamN2
(5'-GCGGATCCCTATGCATACACCTGCATGTTGCTG-3').
Please note that Addgene sequencing results confirm the updated domain numbering to be Met 1701-Ala 2473.
Proper citation: RRID:Addgene_20184 Copy
Species: Homo sapiens
Genetic Insert: RalA
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pPs-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: pPs-neo vector based on psp64
Proper citation: RRID:Addgene_20182 Copy
Species: Homo sapiens
Genetic Insert: RalA
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pPs-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: pPs-neo vector based on psp64
Proper citation: RRID:Addgene_20181 Copy
Species: Mus musculus
Genetic Insert: Sox10-MCS1B
Vector Backbone Description: Backbone Size:4909; Vector Backbone:pE1B-C-F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18773071
Proper citation: RRID:Addgene_20239 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Proper citation: RRID:Addgene_20232 Copy
Species: Homo sapiens
Genetic Insert: TNF receptor-associated factor 2
Vector Backbone Description: Backbone Size:6203; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19150425
Proper citation: RRID:Addgene_20230 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: D153E mutation is also present, but it does affect the plasmid.
Proper citation: RRID:Addgene_20235 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4B
Vector Backbone Description: Backbone Size:5608; Vector Backbone:pBacPAK-HFT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: TEV tag ENPYFQG instead of ENLYFQG.
The cDNA used in making dKDM4B(CG33182) constructs has incomplete 3' end. It is because NCBI data has updated the sequence after I made the construct. There is a comment on NCBI page https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=161077032&from=148&to=1920&view=gbwithparts indicates that they replaced a former sequence of CG33182 https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=28573833 with the current 1773bp one.
Proper citation: RRID:Addgene_20234 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:5608; Vector Backbone:pBacPAK-HFT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: TEV tag ENPYFQG instead of ENLYFQG.
Proper citation: RRID:Addgene_20233 Copy
Species: Homo sapiens
Genetic Insert: NRas
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:5607; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20205 Copy
Species: Mus musculus
Genetic Insert: SV40LgT
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:4663; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20204 Copy
Species: Homo sapiens
Genetic Insert: cIAP1 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18846111
Comments: The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen) and the expression plasmid pMC-cIAP1-FL was constructed by inserting the 3′UTR of cIAP1 downstream of CAT in the pMC construct.
Proper citation: RRID:Addgene_20209 Copy
Genetic Insert: shp53/GFP4
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:5158; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19147555
Proper citation: RRID:Addgene_20208 Copy
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