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Species: Homo sapiens
Genetic Insert: NLRP1 UPA-CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163984 Copy
Species: Mus musculus
Genetic Insert: ADAP1
Vector Backbone Description: Backbone Marker:thermo fisher (Invitrogen); Backbone Size:4818; Vector Backbone:pFastBac HTb; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163906 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 UPA-CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163988 Copy
Species: Synthetic
Genetic Insert: Cas9-VPR
Vector Backbone Description: Vector Backbone:PRS415; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163971 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRS423; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163973 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRS423; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163972 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6850; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD122.75, Ligation number L4066.
Proper citation: RRID:Addgene_1640 Copy
Species: Homo sapiens
Genetic Insert: ASC
Vector Backbone Description: Backbone Marker:Addgene #41392; Vector Backbone:pLEX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163977 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Addgene #41392; Vector Backbone:pLEX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163976 Copy
Species: Homo sapiens
Genetic Insert: PKG1 alpha
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16391 Copy
Species: Drosophila melanogaster
Genetic Insert: Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence.
pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCherry: Clonetech
Proper citation: RRID:Addgene_163926 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163992 Copy
Species: Homo sapiens
Genetic Insert: PKG1 beta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The PKG I beta insert aligns with the listed reference sequence after amino acid 90, contains an EF insertion after the starting methionine and I537V mutation.
Proper citation: RRID:Addgene_16394 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163997 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163996 Copy
Species: Enterobacteria phage T4
Genetic Insert: gp32
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_163912 Copy
Species: Synthetic
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:2507; Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_163915 Copy
Species: Enterobacteria phage T4
Genetic Insert: uvsY
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_163914 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
References:
Comments: Optimized T2A sequence: https://doi.org/10.6084/m9.figshare.13636142.v79.
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) (#137864, https://www.addgene.org/137864/) was updated with (1) improved Kozak consensus sequence, (2) a slightly different codon usage in the 5' linker of the T2A, (3) a silently mutated XhoI site in iCre was reverted, and (4) four nucleotides were restored to the 5' of the WPRE.
Proper citation: RRID:Addgene_177308 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4320; Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Plasmid contains a C/T mixed base causing possible A50V mutation in URA3. This discrepancy is not known to affect plasmid function.
Proper citation: RRID:Addgene_177269 Copy
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