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Species: Homo sapiens
Genetic Insert: hIL6-rens.1
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39814991
Proper citation: RRID:Addgene_231197 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:pPB-cT3G-cERP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2024.05.31.596483 for bioRxiv preprint.
Proper citation: RRID:Addgene_222528 Copy
Species: Homo sapiens
Genetic Insert: DDX4
Vector Backbone Description: Vector Backbone:pPB-cT3G-cERP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2024.05.31.596483 for bioRxiv preprint.
Proper citation: RRID:Addgene_222527 Copy
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39874383
Proper citation: RRID:Addgene_217443 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting IRF3
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39874383
Proper citation: RRID:Addgene_217446 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting PQBP1
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39874383
Proper citation: RRID:Addgene_217447 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Vector Backbone:pCS2-GFP-IRES-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37478862
Comments: Myc numbering based on sequence for c-myc 2.
Proper citation: RRID:Addgene_231236 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting cGAS
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39874383
Proper citation: RRID:Addgene_217444 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Vector Backbone:pCS2-GFP-IRES-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37478862
Comments: Myc numbering based on sequence for c-myc 2.
Proper citation: RRID:Addgene_231235 Copy
Species: Synthetic
Genetic Insert: Alpha-ketoglutarate-dependent Dioxygenase
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33820973
Comments: Please note that this plasmid runs as a dimer. Dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_228218 Copy
Species: Synechocystis sp. PCC 6803
Genetic Insert: slr0214
Vector Backbone Description: Backbone Size:3446; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39846505
Comments: Please visit https://doi.org/10.1101/2024.09.10.611283 for bioRxiv preprint.
Proper citation: RRID:Addgene_226793 Copy
Species: Synthetic
Genetic Insert: dCas9-PmCDA1, sfGFP and sacB
Vector Backbone Description: Vector Backbone:pGGPK-AG2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41415724
Comments: sfGFP and scaffold was amplified from: pEN-L1-PJ23119-Bsa1-PglpT-sfGFP-TrrfB-Bsa1-Scaf-L2
sacB was amplified from: pEN-R2-SacB-L3.
The base editor expression unit was amplified from: pEN-L4-PvirB-dCas9-CDA-UL-T3T-R1.
Please visit https://doi.org/10.1101/2024.08.04.606528 for bioRxiv preprint.
Proper citation: RRID:Addgene_222079 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pHDR; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2024.05.31.596483 for bioRxiv preprint.
Proper citation: RRID:Addgene_222519 Copy
Species: Homo sapiens
Genetic Insert: SumIL2-rens-3GS-IL2Rb
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39814991
Proper citation: RRID:Addgene_231223 Copy
Species: Homo sapiens
Genetic Insert: SumIL2-5GS-rens-IL2Rb
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39814991
Proper citation: RRID:Addgene_231221 Copy
Species: Synthetic
Genetic Insert: dCas9-PmCDA1, sfGFP, sacB and AeBlue
Vector Backbone Description: Vector Backbone:pVP075; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41415724
Comments: sfGFP and scaffold was amplified from: pEN-L1-PJ23119-Bsa1-PglpT-sfGFP-TrrfB-Bsa1-Scaf-L2.
sacB was amplified from: pEN-R2-SacB-L3.
The base editor expression unit was amplified from: pEN-L4-PvirB-dCas9-CDA-UL-T3T-R1.
AeBlue amplified from Addgene #117846.
Please visit https://doi.org/10.1101/2024.08.04.606528 for bioRxiv preprint.
Proper citation: RRID:Addgene_222080 Copy
Species: Homo sapiens
Genetic Insert: hIL6-rens.1_Del
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39814991
Proper citation: RRID:Addgene_231225 Copy
Species: Synthetic
Genetic Insert: cre recombinase with SV40 NLS
Vector Backbone Description: Backbone Size:5184; Vector Backbone:pAAV-ucp1-Cre-3xmiR122-WPRE-HGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39094948
Proper citation: RRID:Addgene_228408 Copy
Species: Neurospora crassa
Genetic Insert: NIT-9
Vector Backbone Description: Vector Backbone:PMF272; Vector Types:Protein expression in N. crassa; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_228409 Copy
Vector Backbone Description: Backbone Size:4572; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: "Empty" vector for rapid cloning via DNA assembly methods (i.e. Gibson, Hifi, In-fusion, etc.) achieved via linearization by SmaI restriction enzyme cleavage.
Proper citation: RRID:Addgene_228486 Copy
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