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Species: Synthetic
Genetic Insert: IPTG-inducible promoter
Vector Backbone Description: Vector Backbone:pL0M-PU-pLac; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-PU-pLac. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113994 Copy
Species: Synthetic
Genetic Insert: IPTG-inducible T7RNAP promoter
Vector Backbone Description: Vector Backbone:pL0M-PU-pT7lacO; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-PU-pT7lacO. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113993 Copy
Species: Homo sapiens
Genetic Insert: MPS1-dTPR
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569217
Proper citation: RRID:Addgene_114048 Copy
Species: Homo sapiens
Genetic Insert: eGFP-HEC1-8A/S15D (NDC80)
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO - hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621779
Proper citation: RRID:Addgene_114031 Copy
Species: Synthetic
Genetic Insert: Level 2 golden gate cloning vector
Vector Backbone Description: Vector Backbone:pLVC-P1-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-P1-GG cloning site Lv2. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113981 Copy
Species: Homo sapiens
Genetic Insert: GFP tagged - HEC1 -M4
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26068855
Proper citation: RRID:Addgene_114036 Copy
Species: Synthetic
Genetic Insert: Empty
Vector Backbone Description: Vector Backbone:pL1V-Lv1-ColE1-amp; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30692983
Comments: pL1V-Lv1-ColE1-amp. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113980 Copy
Species: Homo sapiens
Genetic Insert: GFP tagged - HEC1 -M2
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26068855
Proper citation: RRID:Addgene_114035 Copy
Species: Homo sapiens
Genetic Insert: LacI-LAP-HEC1
Vector Backbone Description: Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26068855
Proper citation: RRID:Addgene_114033 Copy
Species: Homo sapiens
Genetic Insert: MIS12
Vector Backbone Description: Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24695965
Comments: T2098L mutant of FKBP-rapamycin binding domain of human FRAP. Puromycin is expressed via IRES sequence
Proper citation: RRID:Addgene_114039 Copy
Species: Homo sapiens
Genetic Insert: Human MAD1
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24695965
Proper citation: RRID:Addgene_114038 Copy
Species: Synthetic
Genetic Insert: Kanamycin/neomycin
Vector Backbone Description: Vector Backbone:pLVC-P2-neo; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-P2-neo. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113982 Copy
Species: Synthetic
Genetic Insert: Endlinker/terminator Part 4
Vector Backbone Description: Vector Backbone:pLVC-ELT-4; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-ELT-4. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113988 Copy
Species: Homo sapiens
Genetic Insert: sg2+sg3
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32380416
Proper citation: RRID:Addgene_113970 Copy
Species: Homo sapiens
Genetic Insert: eGFP-d80-HEC1 (NDC80)
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO - puromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621779
Comments: Two amino acids M and I are missing before the start of HEC1 that removes a BclI* restriction site- Unsure if this will affect plasmid function.
Proper citation: RRID:Addgene_114025 Copy
Species: Synthetic
Genetic Insert: iRFP670 sgRNA
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32380416
Proper citation: RRID:Addgene_113973 Copy
Species: Homo sapiens
Genetic Insert: eGFP-HEC1 - 9A (NDC80)
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO - hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621779
Proper citation: RRID:Addgene_114028 Copy
Species: Homo sapiens
Genetic Insert: eGFP-HEC1 - Wildtype (NDC80)
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO - hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621779
Proper citation: RRID:Addgene_114027 Copy
Species: Homo sapiens
Genetic Insert: eGFP-STOP-HEC1
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO - hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621779
Proper citation: RRID:Addgene_114026 Copy
Species: Homo sapiens
Genetic Insert: RASA2
Vector Backbone Description: Backbone Size:6606; Vector Backbone:PCDF1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26502337
Proper citation: RRID:Addgene_114097 Copy
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