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Species: Synthetic
Genetic Insert: human codon optimized AsCas12a with C-terminal NLS and His-tag
Vector Backbone Description: Backbone Marker:Schier Lab (Addgene plasmid # 47327); Vector Backbone:pET-28b-Cas9-His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114069 Copy
Species: Synthetic
Genetic Insert: entry vector for LbCas12a crRNAs
Vector Backbone Description: Backbone Marker:Joung Lab (Addgene plasmid # 42230); Vector Backbone:DR247; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114068 Copy
Species: Synthetic
Genetic Insert: entry vector for AsCas12a crRNAs
Vector Backbone Description: Backbone Marker:Joung Lab (Addgene plasmid # 42230); Vector Backbone:DR247; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114067 Copy
Species: Homo sapiens
Genetic Insert: Mps1-KD
Vector Backbone Description: Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18545697
Proper citation: RRID:Addgene_114066 Copy
Species: Synthetic
Genetic Insert: human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
Vector Backbone Description: Backbone Marker:Schier Lab (Addgene plasmid # 47327); Vector Backbone:pET-28b-Cas9-His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114071 Copy
Species: Homo sapiens
Genetic Insert: BUBR1-dKARD
Vector Backbone Description: Backbone Marker:Cheeseman IM and Desai A, Science's STKE 2005;2005:l1; Vector Backbone:pIC58; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23079597
Comments: based on pLAP-BUBR1
Proper citation: RRID:Addgene_114053 Copy
Species: Homo sapiens
Genetic Insert: BUBR1
Vector Backbone Description: Backbone Marker:Cheeseman IM and Desai A, Science's STKE 2005;2005:l1; Vector Backbone:pIC58; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23079597
Proper citation: RRID:Addgene_114052 Copy
Species: Homo sapiens
Genetic Insert: eGFP-HEC1-d207
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569217
Proper citation: RRID:Addgene_114051 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac2(V12) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11397 Copy
Species: Homo sapiens
Genetic Insert: MIS12-KARD-3D
Vector Backbone Description: Backbone Marker:Cheeseman IM and Desai A, Science's STKE 2005;2005:l1; Vector Backbone:pIC58; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23079597
Comments: based on pLAP-MIS12-KARD: point mutations are not included in Sequence:partial 2
Proper citation: RRID:Addgene_114058 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac2(N17) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11398 Copy
Species: Homo sapiens
Genetic Insert: BUBR1-3D
Vector Backbone Description: Backbone Marker:Cheeseman IM and Desai A, Science's STKE 2005;2005:l1; Vector Backbone:pIC58; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23079597
Comments: based on pLAP-BUBR1
Proper citation: RRID:Addgene_114055 Copy
Species: Homo sapiens
Genetic Insert: BUBR1-K795R
Vector Backbone Description: Backbone Marker:Cheeseman IM and Desai A, Science's STKE 2005;2005:l1; Vector Backbone:pIC58; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22698286
Comments: based on pLAP-BUBR1: point mutation(s) are not included in Sequence:Full
Proper citation: RRID:Addgene_114060 Copy
Species: Homo sapiens
Genetic Insert: MIS12
Vector Backbone Description: Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24695965
Comments: T2098L mutant of FKBP-rapamycin binding domain of human FRAP. Puromycin is expressed via IRES sequence
Proper citation: RRID:Addgene_114040 Copy
Vector Backbone Description: Vector Backbone:pL1V-Lv1-gent-pBBR1-ELT3; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:30692983
Comments: pL1V-Lv1-gent-pBBR1-ELT3. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113991 Copy
Species: Homo sapiens
Genetic Insert: MPS1-d60
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569217
Proper citation: RRID:Addgene_114046 Copy
Species: Synthetic
Genetic Insert: Endlinker/terminator Part 6
Vector Backbone Description: Vector Backbone:pLVC-ELT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-ELT-6. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113990 Copy
Species: Homo sapiens
Genetic Insert: MPS1-KD
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569217
Proper citation: RRID:Addgene_114045 Copy
Species: Homo sapiens
Genetic Insert: MPS1-WT
Vector Backbone Description: Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569217
Proper citation: RRID:Addgene_114044 Copy
Species: Synthetic
Genetic Insert: Super-folder GFP
Vector Backbone Description: Vector Backbone:pL0M-SC-sfGFP; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-SC-sfGFP. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113995 Copy
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