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Species: Homo sapiens
Genetic Insert: TEADiv2
Vector Backbone Description: Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39502361
Comments: ENTR vector for Gateway cloning of TEADiv2, GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal. Optimized by a D93E mutation in the YAP1 TBD with respect to original TEADi (Addgene #140144). As with other dominant negative proteins, good levels of expression are necessary for TEADi to block TEADs.
Proper citation: RRID:Addgene_228874 Copy
Species: Homo sapiens
Genetic Insert: TEADiv2
Vector Backbone Description: Backbone Marker:Addgene #44012; Vector Backbone:pInducer20; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39502361
Comments: GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal. Optimized by a D93E mutation in the YAP1 TBD with respect to original TEADi (Addgene #140144). As with other dominant negative proteins, good levels of expression are necessary for TEADi to block TEADs.
Proper citation: RRID:Addgene_228876 Copy
Species: Synthetic
Genetic Insert: Mega' with knockout of GvpB, GvpR and GvpG
Vector Backbone Description: Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39227754
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.07.23.550228v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216945 Copy
Species: Homo sapiens
Genetic Insert: AP1M1 guide
Vector Backbone Description: Backbone Marker:Feng Zhang, Addgene plasmid #62988; Vector Backbone:PX459; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39056144
Proper citation: RRID:Addgene_230030 Copy
Vector Backbone Description: Backbone Marker:Ritola Lab; Vector Backbone:CAG-FRT-MCS-WPRE-SV40 - GCH3; Vector Types:AAV, Cloning Vector; Bacterial Resistance:Ampicillin
Comments: 5' cloning site: BglII (not destroyed), 3' cloning site: AgeI (not destroyed)
Proper citation: RRID:Addgene_230831 Copy
Species: Methanomethylophilus alvus
Genetic Insert: Methanomethylophilus alvus pyrrolysine tRNA synthetase variants
Vector Backbone Description: Backbone Marker:Ryan Mehl (Addgene plasmid # 197573); Vector Backbone:pBK- Ma PylRS WT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36383641
Proper citation: RRID:Addgene_224585 Copy
Species: Homo sapiens
Genetic Insert: GPR137
Vector Backbone Description: Backbone Marker:Addgene #17398; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38103827
Proper citation: RRID:Addgene_226809 Copy
Species: Homo sapiens
Genetic Insert: HCAR3
Vector Backbone Description: Backbone Marker:Addgene #17398; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38103827
Proper citation: RRID:Addgene_226807 Copy
Genetic Insert: n/a
Vector Backbone Description: Backbone Size:6943; Vector Backbone:pGP-Tn7-Cm; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39291982
Proper citation: RRID:Addgene_225282 Copy
Genetic Insert: NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
Vector Backbone Description: Backbone Size:5405; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39557883
Comments: Please visit https://doi.org/10.1101/2022.07.02.498144 for bioRxiv preprint.
Proper citation: RRID:Addgene_226095 Copy
Vector Backbone Description: Backbone Size:6238; Vector Backbone:pGP-Tn7-Cm; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39291982
Proper citation: RRID:Addgene_225281 Copy
Vector Backbone Description: Backbone Size:6943; Vector Backbone:pGP-Tn7-Cm; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39291982
Proper citation: RRID:Addgene_225280 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Backbone Size:4573; Vector Backbone:pMET7-GAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37230591
Proper citation: RRID:Addgene_194455 Copy
Species: Ixodes scapularis
Genetic Insert: 3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis
Vector Backbone Description: Vector Backbone:pGL 4.79 mCherry; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39139404
Comments: Please visit https://doi.org/10.1101/2023.11.29.569248 for bioRxiv preprint.
Proper citation: RRID:Addgene_220091 Copy
Species: T. elongatus
Genetic Insert: kaiB
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_229223 Copy
Species: Mus musculus
Genetic Insert: sg_Trp53_i4
Vector Backbone Description: Vector Backbone:sgTR657_ipUSEPR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38697111
Proper citation: RRID:Addgene_228930 Copy
Species: Mus musculus
Genetic Insert: GSDME
Vector Backbone Description: Vector Backbone:pLentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32188940
Proper citation: RRID:Addgene_223521 Copy
Genetic Insert: PSDΔ1,2-FRBx3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39325885
Proper citation: RRID:Addgene_225827 Copy
Species: Escherichia coli
Genetic Insert: wzxE, wecF, wzyE, wecG
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39772807
Comments: Insert: genes were amplified from E. coli K-12 Sphi874
Proper citation: RRID:Addgene_226782 Copy
Species: Homo sapiens
Genetic Insert: MAP1LC3B
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36814061
Proper citation: RRID:Addgene_228563 Copy
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